Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 4.

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Fig. 4.

Overexpression of the P. ginseng ABA signaling related genes was completely complemented in the ABA insensitive phenotypes of ABA defective Arabidopsis mutants. The ABA-mediated inhibition of seed germination was measured using green cotyledon emergence. Col-0, rcar, rcarPgPYL and snrk2.2/3 PgSnRK transgenic seeds were germinated on 1/2 B5 agar plates containing 0 (A, C) or 1μM of ABA (B, D). Western blot showing HA-tagged PgPYL3, 4 (A), PgSnRK1, 3 (C), PgSnRK1 (E) proteins from independent transgenic lines. Col-0, negative control; CBB staining indicates loading control. Error bars indicate S.E.M. (P<0.05; one-way ANOVA). (E) Relative water loss rates were measured by weight of leaves from 16 days-old Col-0, ost1 (snrk 2.6) and two ost1 PgSnRK1 transgenic lines grown under LD (n = 4). Error bars indicate S.E.M (*P<0.05, Student’s t-test)

J Plant Biotechnol 2018;45:306-14 https://doi.org/10.5010/JPB.2018.45.4.306
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