Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 4.

Download original image
Fig. 4.

Overexpression of the P. ginseng ABA signaling related genes was completely complemented in the ABA insensitive phenotypes of ABA defective Arabidopsis mutants. The ABA-mediated inhibition of seed germination was measured using green cotyledon emergence. Col-0, rcar, rcarPgPYL and snrk2.2/3 PgSnRK transgenic seeds were germinated on 1/2 B5 agar plates containing 0 (A, C) or 1μM of ABA (B, D). Western blot showing HA-tagged PgPYL3, 4 (A), PgSnRK1, 3 (C), PgSnRK1 (E) proteins from independent transgenic lines. Col-0, negative control; CBB staining indicates loading control. Error bars indicate S.E.M. (P<0.05; one-way ANOVA). (E) Relative water loss rates were measured by weight of leaves from 16 days-old Col-0, ost1 (snrk 2.6) and two ost1 PgSnRK1 transgenic lines grown under LD (n = 4). Error bars indicate S.E.M (*P<0.05, Student’s t-test)

J Plant Biotechnol 2018;45:306-14
© 2018 J Plant Biotechnol