Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 1.

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Fig. 1.

Recombinant protein production using the Escherichia coli expression system. Coomassie Brilliant Blue (CBB) R-250 staining clearly showing the successful recombinant expression of (A) 14-3-3s, (B) AtCPK4, CaM2, CaM7, (C) TFL1 and FT. Protein expression induced by adding IPTG to the cell culture for 16 hours at room temperature. Total crude extracts were affinity purified using Ni-NTA resin, separated using 12% SDS-PAGE and stained with CBB

J Plant Biotechnol 2018;45:315-21
© 2018 J Plant Biotechnol