Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 2.

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Fig. 2.

Production and phospho-analysis of cytoplasmic domain (CD) of LRR-RLKs in Escherichia coli expression platform. (A) Analysis of CD of six different Flag-tagged LRR-RLKs (At1g34110, At1g34210, At1g35710, At1g51830, At1g51870, At1g60800) at single times following addition of IPTG (B) Analysis of Flag-At1g34100-CD phosphoprotein production at different induction times after addition of IPTG. For immunoblot analysis, PVDF membranes were probed with phosphothreonine and phosphotyrosine-specific antibodies

J Plant Biotechnol 2018;45:315-21 https://doi.org/10.5010/JPB.2018.45.4.315
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