Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 3.

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Fig. 3.

Autophosphorylation of recombinant Arabidopsis RLCK proteins expressed in Escherichia coli. Anti-phosphothreonine and anti-phosphotyrosine immunoblots showing patterns of autophosphorylation. Not all RLCKs were capable of autophosphorylation following induction in E. coli. Autophosphorylation activity of recombinant proteins was monitored 16 hours after induction of receptor kinase expression. Each sample showed total crude proteins, including FLAG-tagged receptor kinase, extracted from E. coli and subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transfer to polyvinylidene difluoride (PVDF) membranes. Membranes were immunoblotted (IB) using the antibodies indicated

J Plant Biotechnol 2018;45:315-21
© 2018 J Plant Biotechnol