Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 1.

Table. 1.

Troubleshooting conditions for optimization of the phos-tag gel in different running buffers

Experimental conditions Results

Phos – tag (µM) ZnCl2 / MnCl2 (µM) Gel % Running buffer NaHSO3 Gel Electrophoresis After transfer to PVDF membrane
100 200a 6% 1 X Tris- Glycine -b Distorted Distorted
50 100 8% 1 X Tris- Glycine - Distorted Distorted
100 200 8% 1 X Tris- Glycine - Distorted Distorted
100 200 12% 1 X Tris- Glycine - Distorted Distorted
25 50 10% 1 X MOPS +c Partially OK Partially OK
30 60 10% 1 X MOPS + Partially OK Partially OK
50 100 8% 1 X MOPS + Partially OK Partially OK
50 100 12% 1 X MOPS + Partially OK Partially OK
25 50 10% 1 X Tris- Glycine + V. Good V. Good
25 50 12% 1 X Tris- Glycine + Good Good
50 100 10% 1 X Tris- Glycine - Distorted Distorted
100 200 10% 1 X Tris- Glycine + V. Good V. Good

Notes:

MnCl2 was used instead of ZnCl2,

- indicates the absence of NaHSO3 in running buffer during gel electrophoresis,

+ indicates the presence of NaHSO3 in running buffer during gel electrophoresis.

J Plant Biotechnol 2018;45:322-7 https://doi.org/10.5010/JPB.2018.45.4.322
© 2018 J Plant Biotechnol