Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 2.

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Fig. 2.

Detection of plant protein phosphorylation using phos-tag mobility shift assay. (Upper blot) Western blot show the expression level of Protein X. Anti-flag antibody was used for Western blot. (Middle blot) 10% gel containing 100 µM phos-tag run in 1 X Tris-Glycine buffer using previous methods and (Lower blot) 10% gel containing 100 µM phos-tag run in 1 X Tris-Glycine buffer supplemented with 5 mM NaHSO3 using current method. Rubisco was stained using coomassie brilliant blue. 80 µg of total plant protein was loaded in to each well. Osmotic stress caused by 200 mM mannitol treatment was used to induce phosphorylation of protein X in planta

J Plant Biotechnol 2018;45:322-7 https://doi.org/10.5010/JPB.2018.45.4.322
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