Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 4.

Table. 4.

Effect of various media and sucrose concentration on induction of shoot formation from PLB in D. candidum Wallich ex Lindley in vitro

MediaSucrose (g·L-1)Rate of shoot formation (%)Length of shoot (mm)Fresh weight of PLB (g)/ bottle Size of PLB (mm)Browning degree*
1/4 MS1083.3±3.3a1.2±0.2a10.18±1.2bc2.3±0.1bcdef-
2055.0±5.0bcd0.6±0.1a12.05±1.1abc3.1±0.0a-
3035.0±5.0defg1.0±0.2a9.53±0.6cd2.7±0.1abc-

1/2 MS1078.3±1.7ab1.3±0.2a5.74±0.6efg2.3±0.2bcdef-
2046.7±1.7cde1.0±0.3a6.08±0.7def2.0±0.1ef-
3040.0±1.5cdef1.2±0.2a5.31±0.6efg2.2±0.1def-

MS1020.0±0.0fg0.8±0.2a2.09±0.9g1.9±0.1f+++
2020.0±0.0fg1.3±0.1a3.79±0.3fg1.9±0.1f-
3025.0±5.0efg1.5±0.0a2.77±0.4fg1.9±0.1f+++

H1P21081.3±1.3a1.3±0.1a13.46±0.6ab2.6±0.1abcd-
2058.8±1.3bc1.1±0.1a12.01±0.6abc2.8±0.0abc-
3020.0±0.0fg0.7±0.2a5.57±1.3efg2.5±0.1bcde++

H3P41050.0±7.1cd0.9±0.1a15.42±0.7a2.8±0.1ab-
2040.0±0.0cdefg0.8±0.3a8.22±1.2cde2.6±0.1abcde+
3016.0±4.0g1.3±0.4a3.60±0.3fg2.3±0.1cdef+++

- : no browning, +: low, ++: middle, +++: high

Different alphabetical letters are significantly different according to Duncan’s multiple range tests at P < 0.05.

J Plant Biotechnol 2019;46:9-16 https://doi.org/10.5010/JPB.2019.46.1.009
© 2019 J Plant Biotechnol