Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 3.

Download original image
Fig. 3.

Effect of light/dark transition on the saponarin content and related gene expression of young barley seedlings. (a) Expression levels of HvCHS1, HvCHI, and HvOGT1. Fifteen-d-old barley plants grown at 23°C under continuous light conditions were transferred to dark conditions for 1 or 2 d, and the expression levels of HvCHS1, HvCHI, and HvOGT1 in each sample were analyzed using RT–qPCR. The expression levels of each gene at Day 0 were set to 1. Values and error bars indicate mean ± SEM values (n = 3). Asterisks indicate significant differences, when compared to the expression level at Day 0 (Student’s t-test, *P < 0.05, **P < 0.01). n.s., not significant. (b) Saponarin content of light-stressed barley seedlings. The accumulation of saponarin was determined by UHPLC analysis.

J Plant Biotechnol 2019;46:247-54
© 2019 J Plant Biotechnol