Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 5.

Download original image
Fig. 5.

Effect of low and high temperature on the saponarin content and related gene expression of young barley seedlings. (a and b) Expression levels of HvCHS1, HvCHI, and HvOGT1. Fifteen-d-old barley plants grown at 23°C under long day (LD) conditions were transferred to low (4°C, a) or high (37°C, b) temperature conditions for 1 or 2 d, and the expression levels of HvCHS1, HvCHI, and HvOGT1 in each sample were analyzed using RT-qPCR. The expression levels of each gene at Day 0 were set to 1. Values and error bars indicate mean ± SEM values (n = 3). Asterisks indicate significant differences, when compared to the expression level at Day 1 (Student’s t-test, *P < 0.05, **P < 0.01). n.s., not significant. (c and d) Saponarin content of low (4°C, c) and high (37°C, d) temperature-stressed barley seedlings. The accumulation of saponarin was determined by UHPLC analysis.

J Plant Biotechnol 2019;46:247-54
© 2019 J Plant Biotechnol