Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 5.

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Fig. 5.

Effect of low and high temperature on the saponarin content and related gene expression of young barley seedlings. (a and b) Expression levels of HvCHS1, HvCHI, and HvOGT1. Fifteen-d-old barley plants grown at 23°C under long day (LD) conditions were transferred to low (4°C, a) or high (37°C, b) temperature conditions for 1 or 2 d, and the expression levels of HvCHS1, HvCHI, and HvOGT1 in each sample were analyzed using RT-qPCR. The expression levels of each gene at Day 0 were set to 1. Values and error bars indicate mean ± SEM values (n = 3). Asterisks indicate significant differences, when compared to the expression level at Day 1 (Student’s t-test, *P < 0.05, **P < 0.01). n.s., not significant. (c and d) Saponarin content of low (4°C, c) and high (37°C, d) temperature-stressed barley seedlings. The accumulation of saponarin was determined by UHPLC analysis.

J Plant Biotechnol 2019;46:247-54 https://doi.org/10.5010/JPB.2019.46.4.247
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