Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 2.

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Fig. 2.

Phylogenetic tree for CvFatB4, other Cuphea acyl-ACP thioesterases, and UcFatB. The percentage of the tree in which the associated taxa are clustered together in bootstrapping is shown next to the branches. The tree is drawn to scale, with branch lengths representing the number of substitutions per site. The fatty acid most strongly accumulated via heterologous expression is indicated in the parentheses to the right of the protein name. Multiple fatty acids are listed in the parentheses when different fatty acids were dominant in separate experiments. Note that rather broad substrate use was observed in most cases and that fatty acids not indicated in the figure also showed higher accumulation levels compared to the control. NCBI accession numbers, organism(s) used in the heterologous enzyme expression analysis, and relevant references are as follows:1 DQ238595 in Arabidopsis (Filichkin et al. 2006);2 GU225693 in E. coli K27 (Jing et al. 2011);3 U17076 in E. coli and rapeseed (Jones et al. 1995);4 U39834 in E. coli (Dehesh et al. 1996b), Arabidopsis (Tjellström et al. 2013), and rapeseed (Dehesh et al. 1996b);5 KC675178 in Arabidopsis (Tjellström et al. 2013) and Camelina (Kim et al. 2015);6 U38188 in E. coli (Dehesh et al. 1996a);7 U56104 in Arabidopsis (Leonard et al. 1997);8 JF338906 in E. coli (Jing et al. 2011) and Camelina (Kim et al. 2015);9 DQ238594 in Arabidopsis (Filichkin et al. 2006);10 AJ131741 in rapeseed (Beermann et al. 2007);11 JF338908 in E. coli (Jing et al. 2011);12 U38189 in E. coli (Dehesh et al. 1996a) and Arabidopsis (Tjellström et al. 2013);13 KC675179 in Camelina (Kim et al. 2015);14 U56103 in Arabidopsis (Leonard et al. 1997);15 and Q41635 in Arabidopsis (Voelker et al. 1992) and rapeseed (Knutzon et al. 1999). The scale bar shows the fractional differences in the amino acid sequences

J Plant Biotechnol 2019;46:282-90
© 2019 J Plant Biotechnol