Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 3.

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Fig. 3.

Molecular confirmation of the transplastomic lines of Scoparia dulcis L.

a) Confirmation of aadA in transplastomic lines showing a 1.2 kb amplicon; M - marker, Wt - wild type, 1, 2, 3, & 4 - transplastomic lines.

b) Confirmation of the heteroplasmic state showing 2.5 and 3.7 kb amplicons; M - marker, Wt - wild type, 1, 2, & 3 - transplastomic lines.

c) Homoplasmic state confirmation by stable integration of aadA into the plastome of S. dulcis showing 3.7 and 2.5 kb amplicons with PCR in transplastomes and wild type plants, respectively.

d) Southern blotting confirmation of aadA in plastomes showing a 2.55 kb amplicon and absence of the same band in the wild type using the aadA probe.

J Plant Biotechnol 2019;46:323-30 https://doi.org/10.5010/JPB.2019.46.4.323
© 2019 J Plant Biotechnol