Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 4.

Table. 4.

Antimicrobial activities of extracts obtained from leaves and twigs of four species gainst Staphylococcus aureus at varying concentrations according to extraction conditions, compared with those of chemical controls

Part Concentration (mg/disc) Inhibition zone (mm)

Sample (18) Sample (59) Sample (60) Sample (27)
Methylparabena 0.40 -b - - -
1.00 - - - -
2.00 11.0 ± 0.41 gh 11.0 ± 0.41 i 11.0 ± 0.41 g 11.0 ± 0.41 hi
4.00 13.7 ± 0.26 fg 13.7 ± 0.26 f-h 13.7 ± 0.26 e 13.7 ± 0.26 de

Phenoxyethanola 0.40 - - - -
1.00 - - - -
2.00 8.7 ± 0.22 i 8.7 ± 0.22 j 8.7 ± 0.22 i 8.7 ± 0.22 j
4.00 9.9 ± 0.10 hi 9.9 ± 0.10 i 9.9 ± 0.10 gh 9.9 ± 0.10 i
Solvent Extraction time (min)
Leafc MeOH 15 19.5 ± 0.62 b-d 13.4 ± 2.05 f-h 17.5 ± 0.20 c 17.6 ± 0.03 b
30 23.3 ± 0.79 a 13.4 ± 0.56 f-h 21.3 ± 0.64 a 16.6 ± 0.20 bc
45 18.6 ± 0.55 c-e 13.8 ± 0.27 f-h 17.1 ± 0.41 c 17.3 ± 0.58 b

80% EtOH 15 21.8 ± 0.55 ab 12.6 ± 1.80 h 20.6 ± 0.74 ab 16.1 ± 0.50 c
30 21.2 ± 0.55 a-c 13.8 ± 0.58 f-h 19.8 ± 0.54 b 17.2 ± 0.28 bc
45 18.9 ± 0.20 c-e 16.9 ± 0.19 bc 21.3 ± 0.46 a 19.7 ± 0.17 a

DW 15 18.0 ± 0.46 de 14.5 ± 2.14 ef - 12.7 ± 0.75 ef
30 19.6 ± 0.64 b-d 13.9 ± 0.09 fg - 10.8 ± 0.54 hi
45 21.4 ± 1.03 a-c 13.0 ± 0.49 gh - 12.1 ± 0.44 fg
Twigc MeOH 15 11.5 ± 0.50 gh 16.2 ± 0.09 b-d 10.3 ± 0.25 gh 11.5 ± 0.33 gh
30 14.3 ± 0.50 f 15.6 ± 0.56 de 12.1 ± 0.25 f 10.8 ± 0.33 hi
45 18.8 ± 0.18 c-e 16.0 ± 0.31 cd 15.2 ± 0.64 d 14.3 ± 0.19 d

80% EtOH 15 16.4 ± 0.14 ef 17.2 ± 0.22 b 9.4 ± 0.23 hi 14.6 ± 0.32 d
30 14.5 ± 0.14 f 18.5 ± 0.22 a 8.7 ± 0.10 i 18.6 ± 0.10 a
45 18.7 ± 0.29 c-e 17.4 ± 0.40 b 9.3 ± 0.29 hi 19.3 ± 0.18 a

DW 15 - 10.2 ± 0.28 i - 16.1 ± 0.50 c
30 - - - 16.8 ± 0.25 bc
45 - - - 13.8 ± 0.23 de
Part (A) *** *** *** *
Solvent (B) NS *** ** ***
Time (C) * *** ** ***
A × B NS *** *** ***
A × C *** NS *** **
B × C ** *** *** ***
A × B × C NS ** *** ***

Mean ± S.E. (n = 10). Different lowercase letters indicate a significant difference at p < 0.05 based on Duncan's multiple range test.

NS, *, **, ***; nonsignificant or significant at p < 0.05, 0.01, or 0.001, respectively.

aPositive control.

bNot detected.

cConcentration of 2.00 mg/disc was used for all plant extracts

J Plant Biotechnol 2020;47:90-9 https://doi.org/10.5010/JPB.2020.47.1.090
© 2020 J Plant Biotechnol