Fig. 1. Effect of hAβ42 peptide treatment on Arabidopsis mesophyll protoplasts. (a) Morphology of protoplasts treated with 5 µM of hAβ42 monomer and DMSO. 10-d-old Arabidopsis (Col-0) plants were used for isolation of protoplasts, after which 2×104 protoplasts were incubated at 23°C under continuous light conditions and treated with 3 and 5 µM of hAβ42 monomer. After staining protoplasts with Evans blue dye, the dead cells were observed under the microscope (scale bars: 100 µm). (b) Percentage of dead cells at 0, 1, 2, 4, 8, and 24 hours. The asterisks indicate a significant difference in the percentage of dead cells in 3 and 5 µM hAβ42 monomer-treated protoplasts compared with DMSO- treated protoplasts (Student’s t-test, *P < 0.05)
© 2020 J Plant Biotechnol
