Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 4.

Table. 4.

Loci and allelic polymorphism generated by inter-simple sequence repeat markers used for genetic diversity of Thaumatococcus daniellii population from southwestern state of Nigeria

SN Code Sequence (5ˈ - 3ˈ) NAL NML NPL %P FZ (pb)
1 ISSR1 AGAGAGAGAGAGAGAGT 7 3 4 57.1 1300 – 400
2 ISSR2 TGTGTGTGTGTGTGTG 8 3 5 62.5 2000 – 300
3 ISSR3 AGCACGAGCAGCAGCGA 5 3 2 40.0 1400 – 300
4 ISSR4 AGCACGAGCAGCAGCGG 10 4 6 60.0 1400 – 400
5 ISSR5 AGCACGAGCAGCAGCGT 8 5 3 37.5 1100 – 500
6 ISSR6 CACACACACACACAAT 6 1 5 83.3 1700 – 400
7 ISSR7 CACACACACACACAAC 10 4 7 70.0 1800 – 300
8 ISSR8 CACACACACACACAGT 7 3 4 57.1 2000 – 400
9 ISSR9 CACACACACACACAGC 7 2 5 71.4 1500 – 500
10 ISSR10 GTGTGTGTGTGTGTTG 12 4 8 66.7 2800 – 300
11 ISSR11 GTGTGTGTGTGTGTCA 6 3 3 50.0 1600 – 400
12 ISSR12 GTGTGTGTGTGTGTCT 7 2 5 71.4 2500 – 300
13 ISSR13 GTGTGTGTGTGTGTAT 8 2 6 75.0 1000 – 100
14 ISSR14 GCTGAGAGAGAGAGAGA 7 5 2 28.6 1500 – 300
15 ISSR15 GCAGAGAGAGAGAGAGA 5 3 2 40.0 1100 – 300
16 ISSR16 GAGAGAGAGAGACC 9 2 7 77.8 2100 – 300
17 ISSR17 CACACACACACAAG 5 1 4 80.0 1700 – 100
18 ISSR18 CAGCACACACACACACA 5 0 5 100.0 1800 – 500
19 ISSR19 GTGTGTGTGTGTCC 3 0 3 100.0 1400 – 400
Total 136 50 86 63.22
Average 7.16 2.63 4.53 46.28

Keys: NAL: Number of amplified loci; NML: Number of monomorphic loci; NPL: Number of polymorphic loci; %P: Percentage polymorphism; PIC: Polymorphic information content; FZ: Range of amplified fragments

J Plant Biotechnol 2020;47:298-308 https://doi.org/10.5010/JPB.2020.47.4.298
© 2020 J Plant Biotechnol