Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 1.

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Fig. 1. Sequence alignment, PCR products, and high-resolution melting (HRM) curve analysis using the internal transcribed spacer (ITS) nuclear intergenic regions in five Angelica species. (A) Sequence alignment and position of HRM primers of the ITS nuclear intergenic region in each species. The gray box indicates the same sequences in two or three species, whereas the black box indicates the same sequence in all species. Arrows indicate the positions of the ITS primers developed in this study. (B) PCR results using ITS primers. M, marker. (C) Melting curves of ITS from various samples using ITS primers. Experiments for the HRM curve pattern analyses were repeated at least three times with each set of collected samples
J Plant Biotechnol 2021;48:71-6
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