Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 2.

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Fig. 2. Down regulation of β-catenin and TCF4 by RYB in PC-3 cells. (A) PC-3 cells were treated with RYB at the indicated concentrations for 24 hours. (B) PC-3 cells were treated with 200 μg/mL of RYB for the indicated times. (A and B) Cell lysates were subjected to SDS-PAGE and western blot was performed using antibodies against β-catenin and TCF4. β-actin was used as the internal control. Representative blots (left) and quantification of 3 replicates (right) are provided. Values were normalized to the β-actin loading control. Error bars indicate SD. (C and D) RT-PCR analysis of β-catenin and TCF4 gene expression. Total RNA was prepared after RYB treatment at the indicated concentrations for 24 hours (C) or PC-3 cells were treated with 200 μg/mL of RYB for the indicated times (D). GAPDH was used as the internal control. Representative images (left) and quantification of 3 replicates (right) are provided. Values were normalized to the GAPDH loading control. Error bars indicate SD
*p<.05 compared to the cells without RYB treatment
J Plant Biotechnol 2021;48:106-14 https://doi.org/10.5010/JPB.2021.48.2.106
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