Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 4.

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Fig. 4. Effect of RYB on β-catenin phosphorylation in PC-3 cells. (A) PC-3 cells were treated with 200 μg/mL of RYB for the indicated times. Cell lysates were subjected to SDS-PAGE and western blot was performed using antibody against phospho-β-catenin. β-actin was used as an internal control. (B) PC-3 cells were pretreated with the GSK-3β inhibitor LiCl (20 mM) for 2 hours, and then co-treated with RYB (200 μg/mL) for 1 hours. Cell lysates were subjected to SDS-PAGE and western blot was performed using antibody against phospho-β-catenin (Thr286). β-actin was used as an internal control. (A and B) Representative blot (left) and quantification of 3 replicates (right) are provided. All values were normalized to the β-actin loading control. Error bars indicate SD
*P<0.05 compared to cells without RYB treatment
J Plant Biotechnol 2021;48:106-14 https://doi.org/10.5010/JPB.2021.48.2.106
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