Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 5.

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Fig. 5. Functional characterization of PgRGA2 in plants. (A) Plants overexpressing the P gRGA2 gene were rescued the phenotype of the rga quintuple knockout mutants. Scale bar = 3 cm. (B, C) We measured the hypocotyl lengths of the phenotypes. Scale bar = 0.5 cm. Error bars represent the standard error (n = 10). Different lowercase letters indicate statistically significant differences (P < 0.05; one-way ANOVA, followed by Tukey’s multiple-range test). (D) Western blot analysis of proteins extracted from wild-type, rga quintuple-knockout mutant, and PgRGA2-overexpressing rga quintuple-knockout plants. (E) Nuclear localization of PgRGA1-5 proteins in Arabidopsis protoplasts. (F) Exogenous GA3 facilitated PgRGA2 protein degradation. PgRGA2-HA proteins were extracted from the PgRGA2-overexpressing rga quintuple-knockout plants treated with or without 100 μm of GA3 for the indicated time in the presence of cycloheximide
J Plant Biotechnol 2021;48:148-55 https://doi.org/10.5010/JPB.2021.48.3.148
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