Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 1.

Table. 1.

The effect of selective media on frond regeneration and the transformation efficiency of S. polyrhiza turions. Co-cultivation represents

Selective media Co-cultivation Total number of turions Number of transformed cells Transformation efficiency (%)
MS2 16 h light/8 h dark 10 10 90.00±8.16A,#,##
10 8
10 9
Dark 10 8 83.33±4.71*,**
10 8
10 9

MS3 16 h light/8 h dark 10 6 50.00±8.16A
10 5
10 4
Dark 10 5 30.00±16.33
10 3
10 1

Each cultivation group consisted of 10 turions (n = 10). Percentage transformation efficiency is expressed as mean ± standard deviation (SD) of three replicates. Uppercase letters (A) indicate no significant difference between the 16 h light/8 h dark and dark co-cultivation conditions on the same selective medium. The symbol # indicates significant differences on the selective MS2 medium cultivated under 16 h light/8 h dark (# p < 0.05; ## p < 0.01) compared with selective MS3 medium cultivated under 16 h light/8 h dark and dark co-cultivation conditions, respectively. The symbol * indicates significant differences on the selective MS2 medium cultivated under dark (*p < 0.05; **p < 0.01) compared with selective MS3 medium cultivated under 16 h light/8 h dark and dark co-cultivation conditions, respectively.

J Plant Biotechnol 2021;48:156-64 https://doi.org/10.5010/JPB.2021.48.3.156
© 2021 J Plant Biotechnol