Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 1.

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Fig. 1. (A) In vitro multiple shoots (on MS + 3.0 mg/L BAP) that were the source of axenic nodal segments for encapsulation and preparation of synthetic seeds. (B) Excised axenic nodal segments from the primary shoots ready for encapsulation. (C) Production of synthetic seeds by encapsulation of axenic nodal segments using sodium alginate (3%) and CaCl2 (100 mM) with 20 min of ion exchange. (D) Synthetic seeds cultured on ½ MS medium. (E) Sprouting of the synthetic seeds on ½ MS medium. (F) Enlarged view of a sprouted synthetic seed. (G) Germination of synthetic seeds on ½ MS medium (roots indicated by arrow). (H) Magnified view of a single germinated synthetic seed after 14 days of culture. (I) Germinated synthetic seed showing emergence of roots and shoots on ½ MS + 0.5 mg/L IAA after 30 days of culture. (J) Acclimatized plants in pots containing garden soil
J Plant Biotechnol 2021;48:255-63
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