Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 2.

Table. 2.

Mean callus weight of MEsa and ImEsb from four durum wheat varieties obtained on three induction and maintenance culture media after 4 weeks of culturing and their effects on plantlet regeneration (%), NPR/Calc, and NPR/RCald

Callus weight (g) PCIe (%) Plantlet regeneration (%) NPR/Cal NPR/RCal

ME ImE ME ImE ME ImE ME ImE ME ImE
Variety
Amria 2.20f 3.02f 100g 100g 17.54g 26.59f,g 0.40f,g 0.78g 1.97f,g 2.78g
Chaoui 2.06f 3.09f 100g 100g 14.08g 29.85g 0.27f 0.78g 1.42f,g 2.99g
Marouane 2.78g 3.73g 100g 100g 15.29g 36.44g 0.54g 1.06g 2.78g 2.57f
Tomouh 2.20f 2.50h 100g 100g 1.84f 18.33f 0.04h 0.37f 0.60f 1.77f
CDi 0.32 0.46 0 0 4.69 10.12 0.20 0.37 1.37 0.81
Medium*
M1 2.81g 3.06f 100g 100g 13.66f 31.96f 0.39g 0.73f,g 2.26g 2.58g
M2 1.37f 2.12h 100g 100g 19.80g 40.86g 0.50g 1.05g 2.33g 2.70g
M3 2.73g 4.07g 100g 100g 3.09h 10.59h 0.05f 0.45f 0.49f 2.30g
CD 0.27 0.40 0 0 4.06 8.76 0.18 0.32 1.18 0.70

aME: mature embryo.

bImE: immature embryo.

cNPR/Cal: number of plantlets regenerating per callus.

dNPR/RCal: number of plantlets regenerated per regenerating callus.

ePCI: percentage of callus induction.

f,g,hThe values followed by the same letter are not significantly different at α = 0.05 according to Duncan’s multiple-range test.

iCD:

*M4: no callus formation.

J Plant Biotechnol 2022;49:131-8 https://doi.org/10.5010/JPB.2022.49.2.131
© 2022 J Plant Biotechnol