Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 2.

Table. 2.

Effect of various PGRs* on shoot multiplication of AVVP** cultures

PGR Concentration (μM) Shoots Diameter of bulblet (mm) Roots Root length (mm) Existing shoot length (mm) Remarks

Cytokinins Auxin (NAA***)
TDZ **** 0.45 0.0 5.33a 6.09cdefg 1.67d 31.04defg 29.24abcd Shoot cluster formation
4.54 0.0 1.33a 6.14defg 0.67d 9.42i 31.68abcd Shoot cluster inside bulb formation
9.08 0.0 - 10.16a 3.33cd 10.01i 41.95ab Bulb enlargement
22.7 0.0 1.00a 9.79ab 1.67d 17.57hi 39.39ab Bulb enlargement
0.45 0.54 4.00a 7.23cdefg 3.33cd 39.83bcde 17.25cd Shoot cluster formation
4.54 0.54 0.67a 7.75cdefg - - 17.92d Shoot cluster inside bulb formation
9.08 0.54 1.50a 7.93cdefg 1.00d 8.86i 22.76bcd Shoot cluster inside bulb formation
22.7 0.54 - 8.61abc - - 41.66ab Bulb enlargement

Zeatin 0.46 0.0 0.33a 5.69cdefg 4.33bcd 42.34abcd 38.92ab
4.56 0.0 - 6.49cdefg 5.33bcd 25.67efg 38.53abc
9.12 0.0 - 6.11defg 2.33cd 19.24ghi 41.19ab
22.8 0.0 3.00a 6.81cdefg 1.67d 16.38hi 43.85ab Shoot formation
0.46 0.54 - 5.98fg 4.33bcd 41.52abcd 23.18abcd
4.56 0.54 - 5.87fg 2.00d 16.32ghi 34.33abcd
9.12 0.54 5.00a 8.20abcd - - 34.41abcd Shoot formation
22.8 0.54 - 7.17cdefg - - 34.18abcd

2ip 0.49 0.0 - 5.89fg 9.00ab 62.31a 44.57a Root apical tissue formation
4.92 0.0 - 5.79fg 11.33a 53.96abc 32.05abcd Root apical tissue growth
9.84 0.0 0.50a 6.21defg 10.33a 65.67ab 25.38abcd
24.6 0.0 - 6.55cdefg 3.33cd 50.52cd 34.30abcd
0.49 0.54 - 6.09defg 5.00bcd 25.24bcd 34.67abcd Circular root apex
4.92 0.54 - 6.87cdefg 10.33a 45.70a 27.49abcd Growing of root apex
9.84 0.54 - 7.17cdefg 7.33abc 47.87abc 28.43abcd
24.6 0.54 4.00a 8.11bcde 4.00cd 53.97cd 32.60abcd Formation of shoot cluster

*PGR: plant growth regulator.

**AVVP: Allium victorialis var. platyphyllum.

***NAA: 1-naphthaleneacetic acid.

****TDZ: thidiazuron.

Scooped bulbs were cultured for 16 weeks. All experiments were performed in five replications. The values represent the mean, and Duncan's multiple-range test (P ≤ 0.05) was performed.

J Plant Biotechnol 2022;49:213-21 https://doi.org/10.5010/JPB.2022.49.3.213
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