Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Fig. 1.

Download original image
Fig. 1. In vitro multiplication and tuberous root induction in Holostemma annulare. (a) Shoot initiation in Murashige and Skoog (MS) medium supplemented with 0.25 mg/l 6-benzyl adenine (BA). (b) Shoot multiplication in ½ MS medium supplemented with 0.25 mg/l (BA). (c) Shoot initiation in Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg/l BA. (d) Shoot multiplication in SH medium containing 2.0 mg/l BA in combination with 0.5 mg/l kinetin and 1.0 mg/l indole 3-butyric acid. (e) and (f) Tuberous root induction from leaf and shoots, respectively, in SH medium fortified with 0.5 mg/l α-naphthaleneacetic acid. (g) Tuberous roots after 32 days of culture. (h) Root biomass in root suspension culture. (i) Shoot initiation from the in vitro tuberous roots in suspension culture
J Plant Biotechnol 2022;49:230-9
© 2022 J Plant Biotechnol