Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 3.

Table. 3.

Effect of plant growth regulators Schenk and Hildebrandt medium on tuberous root production in Holostemma annulare in vitro shoots

NAA IBA IAA 2,4-D Number of tuberous roots Length of tuberous roots (cm) Diameter of tuberous roots Color of tuberous roots
0.05 - - - 2.12 ± 0.12e 2.29 ± 0.18c 0.13 ± 0.03f Cream
0.1 - - - 3.70 ± 0.15d 3.28 ± 0.19b 0.34 ± 0.04d Cream
0.5 - - - 6.14 ± 0.32a 4.40 ± 0.10a 0.62 ± 0.12a Cream
1.0 - - - 4.52 ± 0.24c 3.50 ± 0.34b 0.53 ± 0.13b Cream
1.5 - - - 3.52 ± 0.35d 3.31 ± 0.22b 0.42 ± 0.08c Cream
2.0 - - - 3.23 ± 0.23d 3.00 ± 0.26b 0.22 ± 0.12e White
- 0.05 - - 1.21 ± 0.12f 0.66 ± 0.15e 0.55 ± 0.17b White
- 0.1 - - 1.03 ± 0.13f 1.57 ± 0.29d 0.15 ± 0.34f White
- 0.5 - - 5.17 ± 0.41b 4.20 ± 0.10a 0.54 ± 0.21b Cream
- 1.0 - - 4.36 ± 0.43c 3.42 ± 0.43b 0.33 ± 0.13d Cream
- 1.5 - - 4.21 ± 0.21c 3.31 ± 0.22b 0.42 ± 0.12c Cream
- 2.0 - - 3.33 ± 0.22d 2.83 ± 0.44c 0.33 ± 0.25d Cream
- - 0.05 - 1.34 ± 0.33f 1.02 ± 0.32d 0.53 ± 0.21b White
- - 0.1 - 1.44 ± 0.22f 1.14 ± 0.11d 0.44 ± 0.11c White
- - 0.5 - 1.45 ± 0.41f 1.17 ± 0.21d 0.34 ± 0.13d White
- - 1.0 - 1.64 ± 0.32f 1.15 ± 0.30d 0.24 ± 0.22e White
- - 1.5 - 1.75 ± 0.43f 1.06 ± 0.32d 0.21 ± 0.41e White
- - - 0.05 1.13 ± 0.32f 0.55 ± 0.43e 0.10 ± 0.30f White
- - - 0.1 1.15 ± 0.14f 0.56 ± 0.24e 0.15 ± 0.05f White
- - - 0.5 1.16 ± 0.32f 0.44 ± 0.33e 0.18 ± 0.21f White
- - - 1.0 1.15 ± 0.17f 0.43 ± 0.36e 0.15 ± 0.11f White
- - - 1.5 1.14 ± 0.42f 0.32 ± 0.21e 0.12 ± 0.01f White
0.1 0.1 - - 2.12 ± 0.37e 1.44 ± 0.25d 0.43 ± 0.41c White
0.5 0.1 - - 3.36 ± 0.23d 1.90 ± 0.04d 0.32 ± 0.32d White
1 0.1 - - 3.75 ± 0.26d 1.80 ± 0.06d 0.41 ± 0.31c Cream
0.5 0.5 - - 4.14 ± 0.19c 2.36 ± 0.21c 0.13 ± 0.23f Cream
0.5 1 - - 3.46 ± 0.27f 2.21 ± 0.17c 0.10 ± 0.24f Cream
1 0.5 - - 4.27 ± 0.43c 2.53 ± 0.15c 0.12 ± 0.32f Cream
1 1 - - 3.66 ± 0.39d 1.93 ± 0.13d 0.33 ± 0.21d Cream
2 0.5 - - 2.16 ± 0.32e 1.42 ± 0.16d 0.24 ± 0.23e Cream
2 1.0 - - 1.44 ± 0.13f 1.36 ± 0.14d 0.42 ± 0.12c Cream

NAA - α-naphthaleneacetic acid; IBA - indole 3-butyric acid; IAA - indole 3-acetic acid; 2,4-D - 2,4-dichlorophenoxyacetic acid

Data represents the mean ± SE of ten replicates, repeated thrice. Mean values followed by the same superscript letter do not differ significantly based on the ANOVA and Duncan’s multiple range test (p ≤ 0.05).

J Plant Biotechnol 2022;49:230-9 https://doi.org/10.5010/JPB.2022.49.3.230
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