Primer sequences used for the qPCR analysis of ADS (amorpha-4,11-diene synthase), CYP (cytochrome P450 mono-oxygenase), and ALDH1 (alcohol dehydrogenase I) genes, which are essential for the biosynthesis of artemisinin
| Primer | sequence (5′ → 3′) | |
|---|---|---|
| ADS | F | ATTACTGGCGGTGCTAAC |
| R | GTGCAGAGACAGCCCATT | |
| CYP | F | ATTACTGGCGGTGCTAAC |
| R | GTGCAGAGACAGCCCATT | |
| ALDH1 | F | CAGGAGCTAATGGAAGTTCTAAGTCAG |
| R | TTTCTTCCTTCGGCCACTGTTG | |
