The target genes were cloned and sequenced to confirm the suitability of the primer designs for ADS (amorpha-4,11-diene hase), CYP (cytochrome P450 mono-oxygenase), and ALDH1 (alcohol dehydrogenase I)
| Target gene | sequence (5′ → 3′) |
|---|---|
| ADS | AATTACTGGCGGTGCTAACCTGCTTACAACATTCTTGTTATCTTGGCATGAGTGATATATTCACAAAAGAGTCTGTCGAATGGGCTGTCTCTGCACA |
| CYP | CTTCCTCATCCACAATCGAATGGGCGATTTCGGAACTCATAAAGTGTCCGAAAGCAATGGAGAAAGTACAAGCGGA |
| ALDH1 | CAGGAGCTAATGGAAGTTCTAAGTCAGCAAGCCATAAAATCAAGTTCACCAAGCTTTTTATCAATGGCGAATTTGTTGATTCTATTTCAGGAAACACTTTTGACACGATTAATCCAGCGACAGAAGAAGTGTTAGCAACAGTGGCCGAAGGAAGAAAA |
