Fig. 4. Gradient PCR was used to amplify the DNA fragments of ITS, matK, and rbcL for barcode analysis. M: 1kb DNA ladder; annealing temperatures of (a) 75°C, (b) 73.5°C, (d) 67.2°C, (f) 58.9°C, and (h) 55°C; (A) Artemisia annua KS; (B) Artemisia annua Danong

J Plant Biotechnol 2022;49:307-15 https://doi.org/10.5010/JPB.2022.49.4.307

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