Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 2.

Table. 2.

Average percentages of cell division and colony formation of hypocotyl protoplasts of 10 A line when cultured with different culture media and plating densities

A. Effects of culture media

Liquid media Cell division (%) Colony formation (%)

14 d 21 d 28 d 28 d 35 d 42 d 50 d

L’4M 20.05b 37.56b 49.13 6.51b 16.58 23.66c 27.09d
L’4M 1 28.93a 45.80a 53.44 9.34a 21.87 30.18ab 34.18b
L’4M 2 32.05a 47.89a 54.25 8.39a 23.82 34.53a 37.44a
L’4M 3 28.36a 46.28a 51.17 8.58a 21.67 29.15b 31.81c

F-test ** ** ns ** ns ** **

B. Effects of plating densities

Plating densities (protoplasts mL-1) Cell division (%) Colony formation (%)

14 d 21 d 28 d 28 d 35 d 42 d 50 d

1×105 29.89a 48.12a 49.88b 3.25b 19.99 27.47b 30.94b
3×105 24.80b 40.65b 54.12a 13.15a 21.98 31.29a 34.32a

F-test ** ** ** ** ns * **

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331
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