Journal of Plant Biotechnology

Table. 2.

Table. 2. Table 2

Average percentages of cell division and colony formation of hypocotyl protoplasts of 10 A line when cultured with different culture media and plating densities

A. Effects of culture media

Liquid media	Cell division (%)			Colony formation (%)

	14 d	21 d	28 d	28 d	35 d	42 d	50 d

L’4M	20.05^b	37.56^b	49.13	6.51^b	16.58	23.66^c	27.09^d
L’4M 1	28.93^a	45.80^a	53.44	9.34^a	21.87	30.18^ab	34.18^b
L’4M 2	32.05^a	47.89^a	54.25	8.39^a	23.82	34.53^a	37.44^a
L’4M 3	28.36^a	46.28^a	51.17	8.58^a	21.67	29.15^b	31.81^c

F-test	**	**	ns	**	ns	**	**

B. Effects of plating densities

Plating densities (protoplasts mL^-1)	Cell division (%)			Colony formation (%)

	14 d	21 d	28 d	28 d	35 d	42 d	50 d

1×10⁵	29.89^a	48.12^a	49.88^b	3.25^b	19.99	27.47^b	30.94^b
3×10⁵	24.80^b	40.65^b	54.12^a	13.15^a	21.98	31.29^a	34.32^a

F-test	**	**	**	**	ns	*	**

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331