Journal of Plant Biotechnology

Table. 3.

Table. 3. Table 3

The effects of culture media and plating densities on cell division at 14, 21, and 28 d, and colony formation at 28, 35, 42, and 50 d of cultured hypocotyl protoplasts of sunflower line PI 441983

Liquid media	Plating densities (protoplasts mL^-1)	Cell division (%)			Colony formation (%)

		14 d	21 d	28 d	28 d	35 d	42 d	50 d
L’4M	1×10⁵	19.42±1.37^b	26.29±1.76^b	37.74±7.29	0.98±0.50	17.10±4.55	22.75±2.95^c	27.30±2.25^b
L’4M	3×10⁵	21.36±0.41^ab	30.63±0.46^ab	42.22±5.50	3.04±1.65	20.54±5.04	29.99±0.75^b	32.91±2.03^ab

L’4M 1	1×10⁵	22.68±1.04^a	34.18±0.99^a	43.43±7.45	2.28±1.16	21.43±4.08	32.26±1.22^ab	35.21±2.53^a
L’4M 1	3×10⁵	22.43±0.30^a	33.37±1.94^a	47.72±5.87	5.41±2.76	25.29±3.65	34.29±0.81^ab	35.28±1.57^a

L’4M 2	1×10⁵	23.45±0.78^a	35.16±1.05^a	43.77±8.06	5.31±2.66	23.43±3.11	32.36±1.12^ab	36.30±2.07^a
L’4M 2	3×10⁵	22.76±0.27^a	37.01±3.02^a	47.09±8.27	5.54±2.95	26.79±3.56	37.47±2.48^a	39.40±1.45^a

L’4M 3	1×10⁵	21.93±0.19^a	37.59±2.21^a	41.13±4.97	4.98±2.64	21.74±2.87	31.02±0.66^b	35.30±2.10^a
L’4M 3	3×10⁵	21.89±0.72^a	37.62±3.39^a	48.45±7.24	5.84±2.94	23.93±2.80	34.52±2.06^ab	37.52±2.56^a

F-test		*	*	ns	ns	ns	**	*

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331