Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 3.

Table. 3.

The effects of culture media and plating densities on cell division at 14, 21, and 28 d, and colony formation at 28, 35, 42, and 50 d of cultured hypocotyl protoplasts of sunflower line PI 441983

Liquid media Plating densities (protoplasts mL-1) Cell division (%) Colony formation (%)

14 d 21 d 28 d 28 d 35 d 42 d 50 d
L’4M 1×105 19.42±1.37b 26.29±1.76b 37.74±7.29 0.98±0.50 17.10±4.55 22.75±2.95c 27.30±2.25b
3×105 21.36±0.41ab 30.63±0.46ab 42.22±5.50 3.04±1.65 20.54±5.04 29.99±0.75b 32.91±2.03ab

L’4M 1 1×105 22.68±1.04a 34.18±0.99a 43.43±7.45 2.28±1.16 21.43±4.08 32.26±1.22ab 35.21±2.53a
3×105 22.43±0.30a 33.37±1.94a 47.72±5.87 5.41±2.76 25.29±3.65 34.29±0.81ab 35.28±1.57a

L’4M 2 1×105 23.45±0.78a 35.16±1.05a 43.77±8.06 5.31±2.66 23.43±3.11 32.36±1.12ab 36.30±2.07a
3×105 22.76±0.27a 37.01±3.02a 47.09±8.27 5.54±2.95 26.79±3.56 37.47±2.48a 39.40±1.45a

L’4M 3 1×105 21.93±0.19a 37.59±2.21a 41.13±4.97 4.98±2.64 21.74±2.87 31.02±0.66b 35.30±2.10a
3×105 21.89±0.72a 37.62±3.39a 48.45±7.24 5.84±2.94 23.93±2.80 34.52±2.06ab 37.52±2.56a

F-test * * ns ns ns ** *

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331
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