Journal of Plant Biotechnology

Table. 4.

Table. 4. Table 4

Average percentages of cell division and colony formation of mesophyll protoplasts of PI 441983 line when cultured with different culture media and plating densities

A. Effects of culture media
Liquid media	Cell division (%)			Colony formation (%)

	14 d	21 d	28 d	28 d	35 d	42 d	50 d

L’4M	20.39^b	28.46^b	39.98	2.01	18.82	^26.37b	^30.11b
L’4M 1	22.55^a	33.78^a	45.58	3.85	23.36	33.28^a	35.25^a
L’4M 2	23.11^a	36.09^a	45.43	5.43	25.11	34.91^a	37.85^a
L’4M 3	21.91^ab	37.61^a	44.79	5.41	22.84	32.77^a	36.41^a

F-test	*	**	ns	ns	ns	**	*

B. Effects of plating densities

Plating densities (protoplasts mL^-1)	Cell division (%)	Colony formation (%)

	14 d	21 d	28 d	28 d	35 d	42 d	50 d
1×10⁵	21.87	33.31	41.52	3.39	20.92	29.60^b	33.53
3×10⁵	22.11	34.66	46.37	4.96	24.14	34.07^a	36.28

F-test	ns	ns	ns	ns	ns	**	ns

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331