Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 4.

Table. 4.

Average percentages of cell division and colony formation of mesophyll protoplasts of PI 441983 line when cultured with different culture media and plating densities

A. Effects of culture media
Liquid media Cell division (%) Colony formation (%)

14 d 21 d 28 d 28 d 35 d 42 d 50 d

L’4M 20.39b 28.46b 39.98 2.01 18.82 26.37b 30.11b
L’4M 1 22.55a 33.78a 45.58 3.85 23.36 33.28a 35.25a
L’4M 2 23.11a 36.09a 45.43 5.43 25.11 34.91a 37.85a
L’4M 3 21.91ab 37.61a 44.79 5.41 22.84 32.77a 36.41a

F-test * ** ns ns ns ** *

B. Effects of plating densities

Plating densities (protoplasts mL-1) Cell division (%) Colony formation (%)

14 d 21 d 28 d 28 d 35 d 42 d 50 d
1×105 21.87 33.31 41.52 3.39 20.92 29.60b 33.53
3×105 22.11 34.66 46.37 4.96 24.14 34.07a 36.28

F-test ns ns ns ns ns ** ns

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331
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