Fig. 1. The development of sunflower protoplasts at 50 d after culturing in various media (L’4M, L’4M 1, L’4M 2, and L’4M 3) and plating densities (1 × 105 and 3 × 105 protoplasts mL-1). (A1-H1) Characteristics of colonies developed from hypocotyl protoplasts of 10 A line. (A2-H2) Visible colonies and microcalli in agarose droplets developed from hypocotyl protoplasts of 10 A line. (I-P) Characteristics of colonies developed from mesophyll protoplasts of PI 441983 line
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