Journal of Plant Biotechnology

Table. 1.

Table. 1. Table 1

The effects of culture media and plating densities on cell division at 14, 21, and 28 d, and colony formation at 28, 35, 42, and 50 d of cultured hypocotyl protoplasts of sunflower line 10 A

Liquid media	Plating densities (protoplasts mL^-1)	Cell division (%)			Colony formation (%)

		14 d	21 d	28 d	28 d	35 d	42 d	50 d
L’4M	1×10⁵	20.59±0.59^cd	39.04±0.92^bc	47.14±0.60^c	2.16 ±0.31^e	15.38±5.93	18.21±0.96^c	24.54±1.58^d
L’4M	3×10⁵	19.51±2.86^d	36.07±3.31^c	51.13±1.49^abc	10.85±0.99^c	17.78±0.37	29.12±1.78^ab	29.64±0.44^c

L’4M 1	1×10⁵	31.49±1.45^ab	50.67±1.59^a	50.68±3.32^abc	3.66±0.60^de	21.51±7.83	28.45±2.53^ab	33.99±1.54^b
L’4M 1	3×10⁵	26.37±3.15^bc	40.93±2.84^bc	56.20±1.00^a	15.01±0.23^a	22.23±1.18	31.91±2.04^ab	34.37±0.90^b

L’4M 2	1×10⁵	35.95±1.85^a	52.03±1.41^a	52.87±0.78^abc	4.32±0.42^d	22.16±6.70	35.70±3.59^a	36.44±0.89^ab
L’4M 2	3×10⁵	28.14±2.02^b	43.76±1.52^b	55.63±2.95^a	12.45±0.71^bc	25.47±0.38	33.36±1.39^ab	38.45±0.85^a

L’4M 3	1×10⁵	31.51±1.75^ab	50.73±3.00^a	48.83±1.08^bc	2.86±0.31^de	20.90±6.77	27.51±2.51^b	28.79±0.92^c
L’4M 3	3×10⁵	25.20±1.58^bcd	41.84±1.21^bc	53.51±1.66^ab	14.31±1.09^ab	22.45±0.27	30.78±2.32^ab	34.82±1.07^b

F-test		**	**	*	**	ns	**	**

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331