Journal of Plant Biotechnology : eISSN 2384-1397 / pISSN 1229-2818

Table. 1.

Table. 1.

The effects of culture media and plating densities on cell division at 14, 21, and 28 d, and colony formation at 28, 35, 42, and 50 d of cultured hypocotyl protoplasts of sunflower line 10 A

Liquid media Plating densities (protoplasts mL-1) Cell division (%) Colony formation (%)

14 d 21 d 28 d 28 d 35 d 42 d 50 d
L’4M 1×105 20.59±0.59cd 39.04±0.92bc 47.14±0.60c 2.16 ±0.31e 15.38±5.93 18.21±0.96c 24.54±1.58d
3×105 19.51±2.86d 36.07±3.31c 51.13±1.49abc 10.85±0.99c 17.78±0.37 29.12±1.78ab 29.64±0.44c

L’4M 1 1×105 31.49±1.45ab 50.67±1.59a 50.68±3.32abc 3.66±0.60de 21.51±7.83 28.45±2.53ab 33.99±1.54b
3×105 26.37±3.15bc 40.93±2.84bc 56.20±1.00a 15.01±0.23a 22.23±1.18 31.91±2.04ab 34.37±0.90b

L’4M 2 1×105 35.95±1.85a 52.03±1.41a 52.87±0.78abc 4.32±0.42d 22.16±6.70 35.70±3.59a 36.44±0.89ab
3×105 28.14±2.02b 43.76±1.52b 55.63±2.95a 12.45±0.71bc 25.47±0.38 33.36±1.39ab 38.45±0.85a

L’4M 3 1×105 31.51±1.75ab 50.73±3.00a 48.83±1.08bc 2.86±0.31de 20.90±6.77 27.51±2.51b 28.79±0.92c
3×105 25.20±1.58bcd 41.84±1.21bc 53.51±1.66ab 14.31±1.09ab 22.45±0.27 30.78±2.32ab 34.82±1.07b

F-test ** ** * ** ns ** **

Means with different letters within the same column are significantly different (p < 0.05) based on Duncan’s multiple range test

J Plant Biotechnol 2022;49:331-8 https://doi.org/10.5010/JPB.2022.49.4.331
© 2022 J Plant Biotechnol