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Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene
J Plant Biotechnol 2017;44:364-371
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Pravin Kumar1・Kulwinder Kaur2・Ram Singh Purty1・Madan Mohan3・Pradeep Kumar Burma4

1University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16C, Dwarka, New Delhi-110078, India
2Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India
3Department of Plant Molecular Biology University of Delhi South Campus, Benito Juarez Road New Delhi-110021, India
4Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India
Correspondence to: M. Mohan (⊠)
Department of Plant Molecular Biology University of Delhi South Campus, Benito Juarez Road New Delhi-110021, India
e-mail: mohanm98@hotmail.com
P. K. Burma (⊠)
Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India
e-mail: pburma@south.du.ac.in
Both author contributed equally.
Received August 31, 2017; Revised November 17, 2017; Accepted November 29, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.
Keywords : Hybrid seed production, male sterile lines, barnase, barstar, tapetum specific promoter, Oryza sativa


December 2017, 44 (4)
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