search for




 

Efficiency of virus elimination in apple calli (cv. Hongro) derived from meristem culture of dormant buds
J Plant Biotechnol 2017;44:379-387
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Mi Young Kim・Jae An Chun・Kang Hee Cho・Seo Jun Park・Se Hee Kim・Han Chan Lee

Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea
Correspondence to: H. C. Lee (⊠)
Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea
e-mail: l0hc0811@korea.kr
Received September 18, 2017; Revised October 17, 2017; Accepted October 23, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Various sizes (0.2~1.2 mm) and developmental stages (referred to as Stage 1~3) of apical and lateral meristems were excised, together or separately, directly from dormant buds of apple ‘Hongro’. They were mixed infected by Apple scar skin viroid (ASSVd), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV), which are major viruses attacking apples. A total of 31 callus lines (> 10 mm in diameter) were obtained by culturing the explants on Murashige and Skoog (MS) medium supplemented with 3% sucrose, 3.0 mg/L benzyladenine (BA) and 0.1 mg/L indole-3-butyric acid (IBA), and they were subjected to RT-PCR analysis for virus detection. A high rate of virus elimination (expressed as the percentage of calli that did not amplify during RT-PCR, i.e., RT-PCR negative calli per total number of calli obtained) was achieved for ACLSV (100%), ASSVd (93.7%), and ASPV (93.7%), whereas it was only 25.8% for ASGV. ASPV was detected in the presence of 2~3 bracts. Simultaneous virus elimination of ASSVd, ASPV, ACLSV, and ASGV occurred during the meristem culture, in which the early stages of the dormant buds (Stage 1) were used, because ASGV was mostly eliminated during that stage. The results of the present study will be valuable for the production of virus-free apple trees.
Keywords : Tissue culture, Meristem excision, Apple tree, Virus detection, RT-PCR


December 2017, 44 (4)
Full Text(PDF) Free

Social Network Service
Services

Cited By Articles
  • CrossRef (0)

Funding Information
  • CrossMark
  • Crossref TDM