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Effect of explant parts and medium components on in vitro regeneration in Osmunda cinnamomea var. forkiensis
J Plant Biotechnol 2017;44:448-453
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Hyuk Joon Kwon1・So Lim Shin1・Cheol Hee Lee2・Soo-Young Kim1

1National Institute of Biological Resources, Incheon 22689, Korea
2Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University
Correspondence to: S.-Y. Kim (⊠)
National Institute of Biological Resources, Incheon 22689, Korea
e-mail: sy7540@korea.kr
These authors contributed equally to this work.
Received June 7, 2017; Revised October 19, 2017; Accepted October 23, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and NaH2PO4 concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to 50 mg・L-1 NaH2PO4 in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, 50 mg・L-1 NaH2PO4, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.
Keywords : Fern, NaH2PO4, Masspropagation, Pteridophyta, Sporophyte


December 2017, 44 (4)
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