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Expression pattern of low-temperature-related genes by the treatment of AFP in the cryopreservation of potato shoot tips
J Plant Biotechnol 2017;44:478-483
Published online December 31, 2017
© 2017 The Korean Society for Plant Biotechnology.

Ji-Hyang Seo1・Su Min Jeon1・Aung Htay Naing1・Jeung Sul Han1・In Hwan Park2・Chang-Kil Kim1

1Department of Horticulture, Kyungpook National University, Daegu 41566, Korea
2Department of Landscape Architecture, Kyungpook National University, Daegu 41566, Korea
Correspondence to: C.-K. Kim (⊠)
Department of Horticulture, Kyungpook National University, Daegu 41566, Korea
e-mail: ckkim@knu.ac.kr
Received December 11, 2017; Revised December 18, 2017; Accepted December 18, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The expression profiles of low-temperature-related genes were examined in in vitro potatoes exposed to a cold condition for 1-3 days. The expression levels of PPI1 and CI21B genes were lineraly elevated from day 1 to day 3, while the opposite trend was observed for CBF4 and CI21A. In addition, the expression of the genes CI21A and CI21B varied, along with specific tissues (leaf, stem, and tuber) and the treatment periods. Therefore, potato shoot tips were cryopreserved with LS and PVS2 containing different oncentrations of AFP. It can thus be inferred that the presence of AFP in LS and PVS2 was likely to elevate expression pattern of the genes. Furthermore, the concentration of AFP (1,500 ng/ml for LS and 500 ng/mg for PVS2) was the best for the cryopreservation of potatoes.
Keywords : Real-time PCR, Vitrification, LS, PVS2


December 2017, 44 (4)
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