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Sugar content and expression of sugar metabolism-related gene in strawberry fruits from various cultivars
J Plant Biotechnol 2018;45:90-101
Published online June 30, 2018
© 2018 The Korean Society for Plant Biotechnology.

Jeongyeo Lee1, Hyun-Bi Kim1, Young-Hee Noh1, Sung Ran Min1, Haeng-Soon Lee1, Jaeeun Jung1, Kun-Hyang Park1, Dae-Soo Kim1, Myeong Hyeon Nam2, Tae Il Kim2, Sun-Ju Kim3*, HyeRan Kim1*

1Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2Nonsan Strawberry Experiment Station, Chungcheong Nam- Do Agricultural Research and Extension Services, Nonsan-si, Chungnam-do, 32914, Republic of Korea
3Department of Bio-Environmental Chemistry, Chungnam National University, Daejeon 34134, Republic of Korea
Correspondence to: H. Kim (✉) e-mail: hrkim@kribb.re.kr
S.-J. Kim (✉) e-mail: kimsunju@cnu.ac.kr
Received May 28, 2018; Revised June 11, 2018; Accepted June 11, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Strawberry (Fragaria × ananassa) is a globally-cultivated and popular fruit crop, prized for its flavor and nutritional value. Sweetness, a key determinant of fruit quality, depends on the sugar composition and concentration. We selected eight strawberry cultivars based on the fruit soluble solids content to represent high and low sugar content groups. The average soluble solid content was 13.6 °Brix (Okmae, Geumsil, Aram, and Maehyang) and 2.9 °Brix (Missionary, Camino Real, Portola, and Gilgyung53), for the high and low sugar content groups, respectively. Sucrose was the main sugar in the cultivars with high sugar content, whereas fructose was the main component in the low sugar content cultivars. Fruit starch concentration ranged from 3.247±0.056 to 3.850±0.055 g/100g, with a 12% higher concentration in the high sugar content cultivars. Additionally, we identified 41 sugar metabolism-related genes in Fragaria × ananassa and analyzed the relationship between their transcripts and the sugar accumulation in fruit. FaGPT1, FaTMT1, FaHXK1, FaPHS1, FaINVA-3, and FacxINV2-1 were highly expressed in the high sugar content cultivars, while FapGlcT, FaTMT2-1, FaPHS2-1, FaSUSY1-1, and FaSUSY1-2 were highly expressed in the low sugar content cultivars. In general, a greater number of genes encoding sugar transporters or involved in sugar synthesis were highly expressed in the high sugar content cultivars. Contrarily, genes involved in sugar degradation were preferentially transcribed in the low sugar content cultivars. Although gene expression was not perfectly proportional to sugar content or concentration, our analysis of the genes involved in sugar metabolism and accumulation in strawberries provides a framework for further studies and for the subsequent engineering of sugar metabolism to enhance fruit quality.
Keywords : Soluble sugar, Glucose, Fructose, Sucrose, Fragaria × ananassa


June 2018, 45 (2)
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