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Cryopreservation of Hevea brasiliensis zygotic embryos by vitrification and encapsulation-dehydration
J Plant Biotechnol 2018;45:333-339
Published online December 31, 2018
© 2018 The Korean Society for Plant Biotechnology.

Korakot Nakkanong, Charassri Nualsri

Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand
Correspondence to: e-mail: korakot_nick@yahoo.com
Received March 12, 2018; Revised July 9, 2018; Accepted July 10, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The mature zygotic embryos of the Hevea brasiliensis were cryopreserved through the use of the vitrification and encapsulation/dehydration techniques. In all the experiments, the zygotic embryos were pre-cultured for three days in the MS medium supplemented with 0.3 M sucrose before they were used for the cryopreservation technique. In the vitrification procedure, the effect of the plant vitrification solutions (PVS2 and PVS3) and exposure time were studied. The highest survival rate (88.87%) and regrowth (66.33%) were achieved when the precultured zygotic embryos were incubated in a loading solution for 20 minutes at 0°C. They were subsequently exposed to PVS2 for 120 minutes at 0°C and plunged directly into liquid nitrogen. Cryopreservation by the encapsulation-dehydration method was successfully done by leaving the encapsulated zygotic embryos in a laminar flow for 4 hours prior to plunging into a LN. The survival rate and regrowth of the encapsulated zygotic embryos were 37.50% and 27.98%, respectively. The cryopreserved zygotic embryos were able to develop into whole plants.
Keywords : zygotic embryo, Hevea brasiliensis, cryopreservation, vitrification, encapsulation-dehydration


December 2018, 45 (4)
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