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Establishment of tissue culture and acclimatization method for in vitro mass propagation of Echeveria laui and Echeveria elegans
J Plant Biotechnol 2019;46:22-31
Published online March 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Youn Hee Kim, Gee Young Lee, Hye Hyeong Kim, Jae Hong Lee, Jae Hong Jung and Sang Deok Lee

Cactus and Succulents Research Institute, GARES, Goyang-si, Gyeonggi-do, Republic of Korea
Horticultural Research Division, GARES, Hwaseong, 18388, Korea
Correspondence to: e-mail: sky3884@gg.go.kr
Received March 18, 2019; Revised March 21, 2019; Accepted March 21, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The objective of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shooting and rooting Echeveria laui and Echeveria elegans for in vitro mass production. To determine the suitable plant parts for callus induction, the leaves were divided into upper, medium and bottom parts and cultured on MS medium at different concentrations with 0 ~ 2 mgL-1 NAA and 0 ~ 4 mgL-1 BA. The upper and middle parts of leaves both showed 100% callus formation rate with NAA 1 mgL-1 and BA 1 mgL-1 treatment in E. laui. The middle parts of leaves showed 83.3% callus formation rate at NAA 2 mgL-1 and BA 4 mgL-1 treatment in E. elegans. The shoot induction rate from callus was highest at NAA 0.1 mgL-1 and BA 3 mgL-1 treatment in E. laui and NAA 0.3 mgL-1 in E. elegans. In addition, the number of shoots formation was 10.4 shoots high in NAA 1 mg L-1 and BA 1 mgL-1 treatment in E. laui and 12.0 shoots in most effective NAA 1 mgL-1 and BA 0.1 mg L-1 treatment in E. elegans. In the case of acclimatization of regenerated plant, growth characteristics did not show any significant difference (35 ~ 55%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate considered plant height and appearance preference of E. laui and E. elegans. It was established that the optimization of culture condition was responsible for the mass propagation in vitro cultures of E. laui and E. elegans.
Keywords : Acclimatization, Echeveria, Growth regulators, Propagation, Regenerated shoot


March 2019, 46 (1)
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