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Culture condition for gametophyte and sporophyte masspropagation of bamboo fern (Coniogramme japonica) using tissue culture
J Plant Biotechnol 2019;46:119-126
Published online June 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Kyungtae Park · Bo Kook Jang · Cheol Hee Lee

Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, Republic of Korea,
Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, Cheongju, Republic of Korea
Correspondence to: e-mail:
Received March 18, 2019; Revised May 22, 2019; Accepted May 22, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study was conducted to investigate the optimal culture method for gametophyte and sporophyte propagation in Coniogramme japonica (Thunb.) Diels, which can be used in various fields. The propagation of prothallus were cultured in 1/4 - 1 Murashige and Skoog medium and Knop medium for 10 weeks. The results indicated that the fresh weight of prothallus was the highest (14.5 g) in 1MS medium. Subsequently, various concentrations of sucrose, activated charcoal and nitrogen source were also added to 1MS medium and cultured for 8 weeks. The results provided that the sucrose concentration was 3% and the fresh weight of prothallus was the highest 10.8 g. According to the concentration in the range of 8.8 ~ 10.8 g, in the case of activated charcoal, the four treatments showed no significant difference. The nitrogen source was added at a concentration of 30, 60 and 120 mM with the ratio of NH4+ : NO3- being 1 : 2. As a result, the fresh weight of all treatments increased to similar level but there was no significant difference. We investigated sporophyte formation according to soil type and the highest number of sporophytes at 228.0 was formed in soils mixed with horticultural substrate and decomposed granite at 2 : 1 (v : v). On the other hand, sporophyte was not formed in soils containing peatmoss except for the one with peatmoss and decomposed granite at 2 : 1 (v : v).
Keywords : Activated Charcoal, Coniogramme japonica (Thunb.) Diels, Culture Media, Fern, Pteridaceae, Sucrose

June 2019, 46 (2)
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