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Effect of salicylic acid and yeast extract on curcuminoids biosynthesis gene expression and curcumin accumulation in cells of Curcuma zedoaria
J Plant Biotechnol 2019;46:172-179
Published online September 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Truong Thi Phuong Lan · Nguyen Duc Huy · Nguyen Ngoc Luong · Hoang Tan Quang · Trinh Huu Tan · Le Thi Anh Thu · Nguyen Xuan Huy · Nguyen Hoang Loc

University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen St., Hue, Vietnam
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
University of Education, Hue University, 34 Le Loi St., Hue, Vietnam
Correspondence to: e-mail: nhloc@hueuni.edu.vn
Received February 1, 2019; Revised May 21, 2019; Accepted July 10, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
Keywords : Curcuma zedoaria, curcuminoid genes, elicitor, salicylic acid, yeast extract


September 2019, 46 (3)
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  • Hue University, Vietnam
     
      DHH 2016-04-56
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