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Transcriptome analysis of a medicinal plant, Pistacia chinensis
J Plant Biotechnol 2019;46:274-281
Published online December 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Ki-Young Choi · Duck Hwan Park · Eun-Soo Seong · Sang Woo Lee · Jin Hang · Li Wan Yi · Jong-Hwa Kim · Jong-Kuk Na

Department of Controlled Agriculture, Kangwon National University, Chuncheon, Kangwon 24341, Republic of Korea
Division of Bioresource Sciences, Kangwon National University, Chuncheon, Kangwon 24341, Republic of Korea
Department of Medicinal Plants, Suwon Women’s University, Suwon 18333, Republic of Korea
International Biological Material Research Center, KRIBB, Daejeon 34141, Republic of Korea
Yunnan Academy of Agricultural Sciences, Yunnan 650223, China
Department of Horticulture, Kangwon National University, Chuncheon, Kangwon 24341, Republic of Korea
Correspondence to: e-mail: jongkook@kangwon.ac.kr
Received November 12, 2019; Revised December 2, 2019; Accepted December 2, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Pistacia chinensis Bunge has not only been used as a medicinal plant to treat various illnesses but its young shoots and leaves have also been used as vegetables. In addition, P. chinensis is used as a rootstock for Pistacia vera (pistachio). Here, the transcriptome of P. chinensis was sequenced to enrich genetic resources and identify secondary metabolite biosynthetic pathways using Illumina RNA-seq methods. De novo assembly resulted in 18,524 unigenes with an average length of 873 bp from 19 million RNA-seq reads. A Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation tool assigned KO (KEGG orthology) numbers to 6,553 (36.2%) unigenes, among which 4,061 unigenes were mapped into 391 different metabolic pathways. For terpenoid backbone and carotenoid biosynthesis pathways, 44 and 22 unigenes encode enzymes corresponding to 30 and 16 entries, respectively. Twentytwo unigenes encode proteins for 16 entries of the carotenoid biosynthesis pathway. As for the phenylpropanoid and flavonoid biosynthesis pathways, 63 and 24 unigenes were homologous to 17 and 14 entry proteins, respectively. Mining of simple sequence repeat identified 2,599 simple sequence repeats from P. chinensis unigenes. The results of the present study provide a valuable resource for in-depth studies on comparative and functional genomics to unravel the underlying mechanisms of the medicinal properties of Pistacia L.
Keywords : Transcriptome, Medicinal plant, Pistacia


December 2019, 46 (4)
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