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Overexpression of Cuphea viscosissima CvFatB4 enhances 16:0 fatty acid accumulation in Arabidopsis
J Plant Biotechnol 2019;46:282-290
Published online December 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Jinouk Yeon · Jong-Sug Park · Sang Ho Lee · Kyeong-Ryeol Lee · Hankuil Yi

Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea
Department of Agricultural Biotechnology, National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea
Division of Biomedical Engineering & Health Science Management, Mokwon University, Daejeon, 35349, Republic of Korea
Correspondence to: e-mail: hankuil.yi@cnu.ac.kr
e-mail: realdanny@korea.kr
Received July 23, 2019; Revised December 9, 2019; Accepted December 9, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Cuphea viscosissima plants accumulate mediumchain fatty acids (MCFAs), i.e., those containing 8 ~ 14 carbons, in their seeds, in addition to the longer carbon chain fatty acids (≥16 carbons) found in a variety of plant species. Previous studies have reported the existence of three C. viscosissima MCFA-producing acyl-acyl carrier protein (ACP) thioesterases with different substrate specificities. In this study, CvFatB4, a novel cDNA clone encoding an acyl- ACP thioesterase (EC 3.1.2.14), was isolated from developing C. viscosissima seeds. Sequence alignment of the deduced amino acid sequence revealed that four catalytic residues for thioesterase activity are conserved and a putative N-terminal chloroplast transit peptide is present. Overexpression of CvFatB4 cDNA, which was under the control of the cauliflower mosaic virus 35S promoter, in Arabidopsis thaliana led to an increase in 16:0 fatty acid (palmitate) levels in the seed oil at the expense of 18:1 and other non-MCFAs.
Keywords : Acyl-acyl carrier protein thioesterase, Cuphea viscosissima, FatB, CvFatB4, 16:0 fatty acid


December 2019, 46 (4)
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