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Effect of light-emitting diode (LED) on in vitro shoot growth and rooting in teak (Tectona grandis L.)
J Plant Biotechnol 2019;46:291-296
Published online December 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Na-Nyum Lee · Ji-Ah Kim· Yong-Wook Kim

Division of Forest Biotechnology, National Institute of Forest Science, Korea
Correspondence to: e-mail: bravekim@korea.kr
Received November 7, 2019; Revised November 26, 2019; Accepted November 26, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study was conducted to determine the effect of a light-emitting diode (LED) on in vitro shoot growth and rooting in teak (Tectona grandis L.). In the experiments with apical bud explants, the greatest shoot elongation (3.2 cm) occurred when they were cultured on DKW medium under 50% blue and 50% red LED mixture (BR), whereas no differences in growth were observed in different light sources (florescent light [F] or BR) or media (MS or DKW). The highest number of shoot multiplication (2.4/explant) or elongation (4.94 cm) was achieved with 0.5 or 1.0 mg/L 6-Benzyladenine (BA) treatment under BR. In addition, the best rooting rate (93.8%) or root length (1.3 cm) was recorded with 0.5 mg/L indole-3-butyric acid (IBA) treatment under BR, and the highest root induction (3.1/explant) was observed in 0.2 mg/L IBA under BR. The in vitro rooted plantlets were hardened and survived well on soil.
Keywords : Light-emitting diode, Micropropagation, Rooting, Shoot multiplication, Tectona grandis L.


December 2019, 46 (4)
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