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Establishment of an efficient regeneration system for Hibiscus syriacus ‘Nanpa’
J Plant Biotechnol 2019;46:297-302
Published online December 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Ji-Hi Son · Hyeon-Jin Sun · Hong-Gyu Kang · Seok-Chul Suh · Hyo-Yeon Lee

Department of Biotechnology, Jeju National University, Jeju, 63243, Korea
Subtropical Horticulture Research Institute, Jeju National University, Jeju 63243, Korea
Department of Molecular Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 63243, Korea
Correspondence to: e-mail:
Received December 2, 2019; Revised December 20, 2019; Accepted December 20, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus ‘Nanpa’ using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shootforming callus production. Our findings show that mature leaves of H. syriacus ‘Nanpa’ can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus ‘Nanpa’ in the future.
Keywords : Callus formation, Hibicus syriacus ‘Nanpa’, Mature leaf, Molecular breeding, Plant regeneration, Shoot induction

December 2019, 46 (4)
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