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In vitro plantlets regeneration by multi-shoots induction and rooting in Chamaecyparis obtusa
J Plant Biotechnol 2019;46:303-309
Published online December 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Ji Ah Kim · Na-Nyum Lee · Yong Wook Kim

Division of Forest Biotechnology, National Institute of Forest Science, Korea
Correspondence to: e-mail:
Received November 11, 2019; Revised November 26, 2019; Accepted November 26, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3- butyric acid.
Keywords : Anti-browning, Chamaecyparis obtuse, Multishoot induction

December 2019, 46 (4)
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