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Optimal Medium Compositions for Plant Regeneration via Adventitious Shoot Formation Using ‘Fuji’ Apple Leaf Explants
J Plant Biotechnol 2019;46:310-317
Published online December 31, 2019
© 2019 The Korean Society for Plant Biotechnology.

Yoon Kyung Lee · Youngju Kwon · Nam-In Hyung

Department of Plant and Food Sciences, Sangmyung University, Cheonan 31066, Korea
Correspondence to: e-mail: nihyung@smu.ac.kr
Received October 1, 2019; Revised October 28, 2019; Accepted October 28, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Plant regeneration protocols for adventitious shoot organogenesis from apple (Malus domestica ‘Fuji’) leaf explants were developed in the present study. The effects of different basal media, types and concentrations of carbon sources, and concentrations of plant growth regulators were evaluated to determine the optimal shoot regeneration conditions for ‘Fuji’ apple leaf explants. On different treatments involving combinations of basal media, LS and N6 media, and different types and concentrations of cytokinins, 6-benzyladenine (BA) and thidiazuron (TDZ), shoot regeneration rates were the highest in the N6 medium combined with BA. Among the plant growth regulator and carbon source combination treatments, 5.0 mg/L BA, and 0.1 mg/L α-naphthalene acetic acid (NAA) with 40 g/L sorbitol was the optimal combination for shoot regeneration. In addition, the optimal sorbitol concentrations for shoot regeneration were 40 g/L and 60 g/L. The highest regeneration (81.8%) was achieved using 40 g/L sorbitol. The regenerated shoots elongated and rooted on rooting medium, consisting of 1/4 MS medium with 0.2 mg/L indole-3-butyric acid (IBA). The plantlets were acclimatized and the regenerated plants exhibited normal phenotypes.
Keywords : Apple, ‘Fuji’, Plant regeneration, Shoot organogenesis, Sorbitol


December 2019, 46 (4)
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