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Correlation of saponarin content with biosynthesis-related gene expression in hulled and hulless barley (Hordeum vulgare L.) cultivars
J Plant Biotechnol 2021;48:12-17
Published online March 31, 2021
© 2021 The Korean Society for Plant Biotechnology.

HanGyeol Lee·Jae-Hyeok Park·A Mi Yoon·Young-Cheon Kim・Chul Soo Park・Ji Yeong Yang·So-Yeun Woo·Woo Duck Seo·Jeong Hwan Lee

Division of Life Sciences, Jeonbuk National University, 567 Baekjedaero, Deokjin-gu, Jeonju, Jeollabuk-do 54896, Republic of Korea
Department of Crop Science and Biotechnology, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do 54896, Republic of Korea
Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea
Correspondence to: e-mail: jhwanlee90@jbnu.ac.kr swd2002@korea.kr
Received January 6, 2021; Revised January 18, 2021; Accepted January 18, 2021.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Saponarin found in young barley sprouts has a variety of beneficial biological and pharmacological properties, including antioxidant, hypoglycemic, antimicrobial, and hepatoprotective activities. Our previous work demonstrated that saponarin content was correlated with the expression levels of three biosynthetic pathway genes [chalcone synthase (HvCHS1), chalcone isomerase (HvCHI), and UDP-Glc:isovitexin 7-O-glucosyltransferase (HvOGT1)] in young barley seedlings under various abiotic stress conditions. In this study, we investigated the saponarin content and expression levels of three saponarin biosynthetic pathway genes in hulled and hulless domestic barley cultivars. In the early developmental stages, some hulled barley cultivars (Kunalbori1 and Heukdahyang) had much higher saponarin contents than did the hulless barley cultivars. An RNA expression analysis showed that in most barley cultivars, decreased saponarin content correlated with reduced expression of HvCHS1 and HvCHI, but not HvOGT1. Heat map analysis revealed both specific increases in HvCHS1 expression in certain hulled and hulless barley cultivars, as well as general changes that occurred during the different developmental stages of each barley cultivar. In summary, our results provide a molecular genetic basis for the metabolic engineering of barley plants to enhance their saponarin content.
Keywords : Hulled and hulless barley cultivars, Chalcone isomerase, Chalcone synthase, Saponarin, UDP-Glc:isovitexin 7-O-glucosyltransferase


March 2021, 48 (1)
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  • Rural Development Administration
     
      PJ01532503, PJ01528303
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