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Silver nitrate and silver-thiosulphate mitigates callus and leaf abscission during Shisham clonal micro-propagation
J Plant Biotechnol 2021;48:173-178
Published online September 30, 2021
© 2021 The Korean Society for Plant Biotechnology.

Manoj Kumar Raturi ・Ajay Thakur

Genetics and Tree Improvement Forest Research Institute, PO: New Forest Dehradun, India 248006
Head and Scientist F, Genetics and Tree Improvement Forest Research Institute, PO: New Forest Dehradun, India 248006
Correspondence to: e-mail: thakura@icfre.org
Received September 13, 2021; Revised September 22, 2021; Accepted September 22, 2021.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Basal callus formation and leaf abscission is a problem in clonal micropropagation. We have described an in vitro clonal propagation protocol of Dalbergia sissoo Roxb (shisham) ‘FRI-14’ in which AgNO3 played important role not only in mitigating problem of leaf abscission and basal callus, but also improved shoot induction and multiplication. Best induction and shoot multiplication was obtained on MS media with 1.5 mg/l 6-BAP and 10 mg/l AgNO3 and half-strength MS media with 0.5 mg/l 6-BAP, 2 mg/l AgNO3 and 50 mg/l Adenine sulphate whereas best ex vitro rooting was obtained with 200 mg/l IBA in pulse treatment.
Keywords : Clonal propagation, Ethylene inhibitors, Tissue-culture, Tahli, Shisham, Callus


September 2021, 48 (3)
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