search for




 

CRISPR/CAS9 as a Powerful Tool for Crop Improvement
J Plant Biotechnol 2017;44:107-114
Published online June 30, 2017
© 2017 The Korean Society for Plant Biotechnology.

Jae-Young Song, Marjohn Niño, Franz Marielle Nogoy, Yu-Jin Jung, Kwon-Kyoo Kang, and Yong-Gu Cho*

Department of Crop Science, Chungbuk National University, Cheongju 28644, Korea,
Department of Horticulture, Hankyong National University, Anseong 17579, Korea
Correspondence to: e-mail: ygcho@cbnu.ac.kr
Received June 4, 2017; Revised June 5, 2017; Accepted June 21, 2017.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical biotechnologies. Earlier generations of knockout and gain-of- function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/ Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

Keywords : Base targeting, CRISPR/Cas9, Genome editing, Crop improvement
Introduction

Recent advances in genome engineering technologies offer tremendous opportunities to accelerate crop functional genomics studies in a bid to provide robust data for crop improvement. Application of nucleases as editing tool has come a long way in modifying genomes not only with model organisms but even those with genetically challenging components. To date, CRISPR/Cas9 system, has emerged as the most effective genome editing tool overcoming the limitations faced by earlier technologies such as the limited choice of targets with ZFN due to context-dependent effects between individual finger domains in an array (Wolfe et al. 2000; Sanders and Joung 2014) and the delivery issues with TALEN for certain viral vectors (Holkers et al. 2013; Sanders and Joung 2014). Unlike the predecessor zinc finger nuclease (ZFN) and TAL effector nuclease (TALEN), which involve dimerizing fusion proteins including the DNA binding domains of ZF and TAL and cleavage domains of FokI endonuclease, Cas9/gRNA is a ribonucleoprotein active on target DNA (Char et al. 2017). This technology has been widely adopted to study important genes in the cell of mice (Mashiko et al. 2014), monkeys (Niu et al. 2014), and other organisms, including bacteria (Fabre et al. 2014), yeast (DiCarlo et al. 2013), zebrafish (Hwang et al. 2013), Drosophila (Gratz et al. 2014), rabbits (Yang et al. 2014), pigs (Hai et al. 2014), rats (Ma et al. 2014), human (Mali et al. 2013), and plants (Mali et al. 2013). In biomedical field, significant applications have already been achieved including correcting human genetic disorder, treatment of the acquired immune deficiency syndrome (AIDS) or promoting anti-tumor immunotherapy, and genetic manipulation of domesticated animals for production of biologic medical materials among others (Cai et al. 2016). While CRISPR/Cas9 application is expected to fast track molecular breeding without retention of transgene components in the plant product, it also faces a number of hurdles obstracting its maximum potential use especially in crops with large, polyploid genomes (Cram et al. 2017). This was dealt by developing powerful complementary bioinformatics tools that would facilitate full implementation of CRISPR/Cas9 technology in plant genetic engineering. Nevertheless, this technology has been successfully applied in several plant systems, in fact quite a number of reports have been published since its discovery. As this technique rapidly evolves, its application is constantly expanding which can be adapted to spur plant breeding for certain traits that are too challenging using conventional techniques.

Principle of CRISPR/Cas9 and creating variation

Genome editing technologies have been developed to induce site-specific DNA cleavage. The site-specific nucleases (SSNs) can induce double-strand break (DSB) specifically in targeted loci of genomic DNA for genetic improvement in any organisms (Jinek et al. 2012; Cong et al. 2013; Mali et al. 2013). The SSNs system can be classified mainly into three classes, which are zinc finger nucleases (ZFNs) (Kim et al. 1996), transcriptional activator-like effector nucleases (TALENs) (Christian et al. 2010), and the CRISPR/ Cas9 nuclease system from Streptococcus pyogenes (Jinek et al. 2012). Among them, the CRISPR/Cas9 system is originated from the immune response system of bacteria which protect themselves by cleaving the DNA of invading viruses (Bortesi and Fischer 2015). To create variation by gene cleavages, Cas9 nuclease and single guide RNA (sgRNA) complex recognizes the protospacer-adjacent motif (PAM), which is 5’-NGG-3’ in the target site and binds a specific genomic location guided by a short sequence (20 bp) in sgRNA, and then Cas9 cleaves target DNA at 3 bp upstream of the PAM (Jinek et al. 2012). The PAM sequence plays an important role in binding and breaking to the target DNA (Sternberg et al. 2014). The CRISPR/Cas system repairs targeted DNA location very specifically by either via the non-homologous end joining (NHEJ) DNA repair pathway (Rouet et al. 1994) or the homology directed repair (HDR) (Bibikova et al. 2002) pathway with cellular DNA repair mechanisms (Abdallah et al. 2015; Luo et al. 2017). DSB repair through the NHEJ can generate gene knock-outs via small insertions or deletions conferring loss-of-function at the repair positions, while HDR can lead to gene knock-ins as precise sequence alterations for gene replacement conferring gain-of-function (Altpeter et al. 2017; Puchta and Fauser 2014; Voytas and Gao 2014) (Fig. 1). In plants, most of the reported gene editing events was mediated by NHEJ repair mechanism to generate mutations and gene knock-outs. However, gene replacement rather than gene inactivation will greatly facilitate and improve plant breeding by allowing the introduction of precise point mutations or enabling new functions. HDR-based repair generated by CRISPR/Cas9 potentially can provide a feasible approach to achieve gene replacement but currently suffers from very low efficiencies (Lowder et al. 2016; Sun 2017).

Fig. 1.

Process showing CRISPR/Cas9 system as a powerful tool for crop improvement. (A) Designing single guide RNA (sgRNA), (B) Engineering CRISPR/Cas9 nucleases with altered protospacer adjacent motif (PAM), (C) Targeting specific cleavage in plant genome, (D) Editing gene by non-homologous end joining (NHEJ) with CRISPR/Cas9, (E) Selecting null segregants in the next generation


Milestones of CRISPR/Cas9 in crop biotechnology

Different Cas9/gRNA systems have been tailored for effective genomic alterations in both prokaryotes and eukaryotes (Char et al. 2017). Following successful demonstration in mammalian cells, this technology has been adapted in some plant species, and accumulating evidences of its feasibility have been reported in rice (Feng et al. 2013; Jiang et al. 2013; Shan et al. 2013; Zhang et al. 2014; Wang et al. 2016), maize (Feng et al. 2015; Svitashev et al. 2015; Zhu et al. 2015; Char et al. 2017), wheat (Shan et al. 2013; Wang et al. 2014), tomato (Brooks et al. 2014; Cermak et al. 2015), soybean (Li et al. 2015), and potato (Wang et al. 2015). Rice, being a diploid and a monocot plant, has been one of the top choices among researchers for CRISPR/Cas9 demonstration. Some of the pioneering works done in rice involved targeting the promoter regions of bacterial blight susceptibility genes, OsSWEET14 and OsSWEET11 (Jiang et al. 2013). In this study, precise editing was mediated by two constructs including S. pyogenes Cas9 enzyme expressed under CaMV 35S and a chimeric sgRNA driven by a rice U6 gene promoter, which contains a 5’ region complementary to a sequence of 20 bp in the promoter of OsSWEET14 and a 3’ sgRNA scaffold that recruits Cas9. These constructs were successfully transformed into rice using PEG-protoplast transfection protocol. The use of this delivery method clearly showed that mutagenesis by Cas9/sgRNA complex indeed occurs within the plant cells which are free of any bacterial cell involvement (Jiang et al. 2013). The group also demonstrated that codon optimization of the Cas9 positively influenced mutagenesis rate, in this case, using the other bacterial blight susceptibility gene, OsSWEET11. Using the same technique down to codon optimization of the Cas9, Shan et al. (2013) targeted four rice genes, phytoene desaturase (OsPDS), betaine aldehyde dehydrogenase (OsBADH2), basic helix–loop–helix (bHLH) transcription factor (Os02g23823), and mitogen-activated protein kinase (OsMPK2), in separate constructs where sgRNA was expressed by U3 promoter. Each of the endogenous genes was specifically disrupted using only single sgRNA, except for OsPDS, which was targeted by two sgRNAs. On average, high mutagenesis frequency rates were observed in both rice protoplast cells and transgenic plants (Table 1). It has been reported that % GC content, targeting strand and targeting context of sgRNA targeting sequences may influence sgRNA efficacy (Wang et al. 2014). This was successfully demonstrated by Zhang et al. (2014), wherein all, except one, of the 11 sgRNAs examined, showed higher editing efficiency. This group investigated the amenability of 11 rice genes to CRISPR/Cas9 system designed to be disrupted singly and in combination using Agrobacterium- mediated delivery (Table 1). It’s commonly known that homozygous or bi-allelic T0 plants occur when both copies of the target gene in rice are mutated before the first division of the embryogenic cell. In the study conducted by Zhang et al. (2014), 7.7 % of the plants carrying targeted mutations were found to contain homozygous mutations, which implies that CRISPR/Cas9 acts early during the regeneration of T0 plants. Further, simultaneous targeting of two genes for four pairs of targets including OsMSH1 and OsDERF1, OsMSH1 and OsPDS, OsPDS and OsPMS3, and OsPDS and OsDERF1 yields high site-specific mutation rates (Table 2) which are not too different from the expected double mutation rates, that is, the mutation rate at one sites times the rate at the other site. These suggest that mutations at the two sites targeted by one construct occurred independently of each other (Zhang et al., 2014). Application of this technology in dicot plants including tomato (Brooks et al. 2014; Cermak et al. 2015), Soybean (Li et al. 2015), and potato (Wang et al. 2015) have also yielded high site-specific target mutations, thus pointing out the feasibility of CRISPR/Cas9 in most, if not all, plant systems indicating its potential to revolutionize the genome editing of economically important crops.

Single gene editing by CRISPR/Cas9 in major crops

CropsTarget GeneGene Product/traitPROMOTERDeliveryMutagenesis rate (%)off-targetReferences
Cas9sgRNAprotoplasttransgenic
RiceOsSWEET11sucrose efflux transporterCaMV 35SOsU6PEGN.A.N.A.Jiang et al. 2013
OsSWEET14N.A.N.A.
OsPDSphytoene desaturase14.5 - 209.4
OsBADH2betaine aldehyde dehydrogenaseCaMV 35SOsU3PEG / Particle bombardment26.57.1YesShan et al. 2013
Os02g23823a basic helix–loop–helix (bHLH) transcription factor26N.A.
OsMPK2a mitogen-activated protein kinase384
ROC5Rice Outermost Cell-specific gene5N.A.25.8
SPPStromal Processing PeptodaseCaMV 35SOsU6-2AgrobacteriumN.A.4.7Feng et al. 2013
YSAYoung Seedling AlbinoN.A.75
OsPDSPhytoene desaturase41.9
OsEPSPSEPSP synthase21.1
OsDERF1AP2 domain containing protein50.9
OsMSH1DNA mismatch repair protein2x CaMV 35SZmU3Agrobacterium37NoneZhang et al. 2014
OsROC5Leucine zipper class IV protein65.1
OsSPPRice stromal processing peptidase28.9
OsYSAPentatricopeptide repeat domain containing protein51.4
OsERF922Ethylene responsive factorsPubiOsU6AgrobacteriumN.A.42Wang et al. 2016

MaizeZmzb7IspH protein for methyl-D-erythritol-4- phosphate (MEP) pathway2x CaMV 35SZmU3Agrobacterium5086Feng et al. 2015
PSY1Phytoene synthaseZmUBI2ZmU6PEG10.67NoneZhu et al. 2015
LIG1liguleless1ZmUBI1ZmU6particle bombardment / AgrobacteriumN.A.3.9
Ms26male fertility genesN.A.1.75Svitashev et al. 2015
Ms45N.A.0.47
ALS1, ALS2acetolactate synthaseN.A.2.23

WheatTaMLOwheat ortholog of barley MLO proteinCaMV 35SOsU3PEG28.5N.A.NoneShan et al. 2013
TaMLO-A1Mildew Resistance Locus proteinUbiU6particle bombardment5.60%Wang et al. 2014

TomatoSlAGO7ARGONAUTE7CaMV 35SAtU6Agrobacterium48Brooks et al. 2014
Solyc08g041770tomato reproductive development75
ANT1anthocyanin mutant1CaMV 35SAtU6Agrobacterium-mediated29Cermak et al. 2015

SoybeanDD20EF1A2GmU6particle bombardment59Li et al. 2015
DD4376

PotatoStIAA2Aux/IAA proteinCaMV 35SStU6PAgrobacterium-mediatedNoneWang et al. 2015

Double gene editing by CRISPR/Cas9 in selected major crops

CropsGeneGene product/TraitDeliveryPROMOTERMutagenesis frequency (%)References
Cas9sgRNATarget 1Target 2Both
MaizeLIG, MS26LIG(liguleless) MS26&45(male sterility)particle bombardment / AgrobacteriumZmUbiZmU61.43% - 1.78%Svitashev et al. 2015
MS26, MS451.62% - 1.67%
LIG, MS26, MS451.55% - 1.86%

RiceOsMSH1, OsDERF1OsMSH1(pleiotrophic phenotype); OsDERF1(drought-resistant); OsPDS(albino); OsPMS3(non-coding RNA)Agrobacterium2x CaMV 35SZmU35056.733.3Zhang et al. 2014
OsMSH1, OsPDS4056.432.7
OsPDS, OsPMS331.417.18.6
OsPDS, OsDERF137.117.15.7

TomatoSolyc07g021170,Solyc12g044760tomato reproductive developmentAgrobacteriumCaMV 35SAtU6100Brooks et al. 2014

Up until now, most of the reported gene editing events in plants is mediated by an error-prone NHEJ repair mechanism, and only limited evidence that HDR-mediated gene insertion works well in plants have been presented so far due to impractically low frequencies. The Cas9-gRNA system was tested for its ability to facilitate targeted gene insertion in maize immature embryo cells (Svitashev et al. 2015). Here, the DNA donor repair template contained the constitutively expressed PAT gene (UBI:MoPAT) flanked by approximately 1.0 K of DNA fragments homologous to genomic sequences immediately adjacent to the Liguless1 (LIG) cleavage site. Interestingly, only particle bombardment yielded target site-specific gene insertion, and that, donor template DNA designed on the same plasmid together with Cas9-sgRNA resulted in doubled high integration event rate compared when components were delivered as separate vectors. However, this does not necessarily mean that HDR does not work through Agrobacterium-mediated delivery but more likely indicates lower frequency of integration events (Svitashev et al. 2015).

Target base editing using modified CRISPR/Cas9 system

Creation of a much more powerful CRISPR tool box has been reported very recently. The new technique called “base editing” was first demonstrated in mammalian cells, which enables direct and irreversible conversion of one target base into another in a programmable manner, without requiring dsDNA DSB or a donor template (Komor et al. 2016). This new system utilized catalytically modified Cas9 (dCas9) fused to a cytidine deaminase enzyme encoded by the rat APOBEC1 gene. dCas9, which possessed Asp10Ala and His840Ala mutations inactivating its nuclease activity, retains its DNA binding ability via guide RNA, but does not cleave the DNA backbone (Jinek et al. 2012). Instead, this cytidine deaminase converts cytosine (C) bases into uridines (U) (Kuscu and Adli 2016), thereby effecting a 15%-75% of C→T (or G→A) substitution in human cells (Komor et al. 2016), which are then repaired by error-prone mechanisms that result in various point mutations. Fusion of rat APOBEC1 to the amino terminus, but not that carboxy terminus of dCas9, is responsible for a preserved deaminase activity (Komor et al. 2016). Early demonstration in human cells by Komor et al. (2016) yielded no detectable base editing at the known dCas9 off-target sites, and that, base editors in human cells do not induce untargeted C→T conversion throughout the genome. Later on, Lu and Zhu (2016) tested the applicability of this modified CRISPR/Cas9 system in the rice callus of Zhonghua11 (ZH11) using Agrobacterium-mediated transformation. In their study, they fused rat APOBEC1 to the N-terminus of Cas9(D10A) using the unstructured 16-residue peptide XTEN as linker, and constructed it into a binary vector containing maize ubiquitin promoter. This system was used to edit two agriculturally important genes in rice, NRT1.1B and SLR1. Previous reports claimed that a C→T variation (Thr327Met) in NRT1.1B could increase nitrogen use efficiency in rice (Hu et al. 2015), while the amino acid substitution in or near TVHYNP of the DELLA protein encoded by SLR1 could reduce plant height (Ikeda et al. 2001; Asano et al. 2009). Results showed similar base-editing results in human cells, wherein respective C→T substitution frequency in NRT1.1B and SLR1 was 1.4%-11.5%, while C→G replacement frequency was at 1.6%-3.9%. Lu and Zhu (2016) declared that the possible cause of the lower base-editing efficiency on NRT1.1B was the lower targeting efficiency of the gRNA for this gene. Furthermore, to demonstrate feasibility of this new approach in plant breeding program, Lu and Zhu (2016) generated stable transgenic seedlings from the hygromacin-resistant callus. Sanger sequencing and RFLP results of SLR1 consistently confirmed the base replacement in the six transgenic lines which showed a semi-dwarf phenotype. The compelling evidence shown by these studies put CRISPR base-editing technology at the forefront of advancing the efficiency and scope of genome editing.

Implications of improved genome editing in current plant breeding programs

Current challenges in agriculture can be summed up to how we can improve crop production in harsh climatic conditions. Abiotic stress such as drought, salinity, heat, cold, flooding, and radiation are major threat in securing high yield especially of major crops across the globe. To cope these challenges, plants resort to induction of complex interactions among various components of several signaling, regulatory and metabolic pathways (Nakashima et al. 2009), thereby involving multiple genes. For the past decades, generation of stress- tolerant crops have been demonstrated using conventional breeding or classical biotechnologies. However, aside from time-constraint, lack of precision proved to be the bottleneck of these classical approaches. Research works for past five years presented compelling evidence that multiplex genome editing is feasible using CRISPR/Cas9 not only in model plants but also in major crops. This provides opportunities to understand the complexity of major traits in crops by deciphering the functions of multiple genes involved in a single trait. Another approach could be the pyramiding of multiple genes involved in a stress response pathway or regulatory network via HDR-mediated gene targeting (Jain 2015). Most known agriculturally important traits, however are conferred by point mutations (Huang et al. 2010). Hence, techniques that enable precise and efficient base replacement in the target locus, rather than stochastic disruption of the gene, will greatly facilitate precision plant molecular breeding (Lu et al. 2016). The development of a new approach, base editing, conferred novel functionality that is more powerful than the former CRISPR/Cas9 version, wherein it can directly convert irreversibly one target DNA base into another without DSB or a donor template (Komor et al. 2016). This platform can be harnessed to induce genetic variation which is a key component for crop improvement programs.

These advances in genome editing also allow breeders to select null segregant lines, which lack the CRISPR/Cas9 component, by selfing (Fig. 1). The resulting product is expected to be identical to the classically bred plants (Giddings et al. 2012; Khatodia et al. 2016).

Acknowledgments

This research was supported by a grant from the Next- Generation BioGreen 21 Program (National Center for GM Crops No. PJ01191601), Rural Development Administration, and from the National Research Foundation (NRF) programs (2014R1A2A1A11052547) funded by the Korean Ministry of Science, ICT and Future Planning, Republic of Korea.

References
  1. Abdallah NA, Prakash CS, and McHughen AG. (2015) 2015 editing for crop improvement:Challenges and opportunities. GM Crops & Food 6, 183-205.
    Pubmed KoreaMed CrossRef
  2. Altpeter F, Kannan B, Jung JH, Oz TM, Karan R, and Merotto A. (2017). 2017 Improvement of Sugarcane by Targeted Loss- or Gain of Function Mutations using TALEN or CRISPR-Cas9 , pp.W699. Proc. of the Plant and Animal Genome Conference (PAG XXV), San Diego, CA, USA.
  3. Asano K, Hirano K, Ueguchi-Tanaka M, Angeles-Shim RB, Komura T, Satoh H, Kitano H, Matsuoka M, and Ashikari M. (2009) 2009 and characterization of dominant dwarf mutants, Slr1-d, in rice. Mol. Genet. Genomics 281, 223-231.
    Pubmed CrossRef
  4. Bibikova M, Golic M, Golic KG, and Carroll D. (2002) 2002 chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics 161, 1169-1175.
  5. Bortesi L, and Fischer R. (2015) 2015 CRISPR/Cas9 system for plant genome editing and beyond. Biotechnology Advances 33, 41-52.
    Pubmed CrossRef
  6. Brooks C, Nekrasov V, Lippman ZB, and Eck JV. (2014) 2014 gene editing in tomato in the first generation using the clustered regularly insterspaced short palindromic repeats/ CRISPR-Associated9 system. Plant Physiology 166, 1292-1297.
    Pubmed KoreaMed CrossRef
  7. Cai L, Fisher AL, Hunag H, and Xie Z. (2016) 2016-mediated genome editing and human diseases. Genes & Diseases 3, 244-251.
    CrossRef
  8. Čermák T, Baltes NJ, Čegan R, Zhang Y, and Voytas DF. (2015) High-frequency. Precise modification of the tomato genome. Genome Biology .
    CrossRef
  9. Char SN, Neelakandan AK, Nahamoun H, Frame B, Main M, Soalding MH, Becraft PW, Meyers BC, Walbot V, Wang K, and Yang B. (2017) 2017 Agrobacterium-delivered CRISPR/Cas9 system for high frequency targeted mutagenesis in maize. Plant Biotechnology Journal 15, 257-268.
    CrossRef
  10. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, and Zhang F. (2013) 2013 genome engineering using CRISPR/Cas systems. Science 339, 819-823.
    Pubmed KoreaMed CrossRef
  11. Cram D, Kulkarni M, Rozwadowski K, Sharpe AG, and Kagale S. (2017). 2017 CRISPR:A Web-Based Optimized sgRNA Designer for CRISPR-Cas9-Mediated Genome Editing in Wheat , P0821.
  12. DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, and Church GM. (2013). 2013 engineering in Saccharomyces cerevisiae using CRISPR-Cas systems 41, 4336-43.
    CrossRef
  13. Fabre L, Le Hello S, Roux C, Jeanjean SI, and Weill FX. (2014) 2014 Is an Optimal Target for the Design of Specific PCR Assays for Salmonella enterica Serotypes Typhi and Paratyphi A. PLoS Negl Trop Dis 8, e2671.
  14. Feng C, Yuan J, Wang R, Liu Y, Birchler JA, and Han F. (2015) 2015 targeted genome modification in maize using CRISPR/Cas9 system. Journal of Genetics and Genomics 43, 37-43.
    Pubmed CrossRef
  15. Feng Z, Zhang B, Ding W, Liu X, Yang DL, Wei P, Cao F, Zhu S, Zhang F, Mao Y, and Zhu JK. (2013) 2013 genome editing in plants using CRISPR/Cas system. Cell Research 23, 1229-1232.
    CrossRef
  16. Giddings LV, Potrykus I, Ammann K, and Fedoroff NV. (2012) 2012 the Gordian knot. Nat.Biotechnol 30, 208-209.
    CrossRef
  17. Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings AM, and O’Connor-Giles KM. (2014) 2014 specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Genetics 196, 961-71.
  18. Hai T, Teng F, Guo R, Li W, and Zhou Q. (2014) 2014-step generation of knockout pigs by zygote injection of CRISPR. Cas system Cell Res 24, 372-375.
    CrossRef
  19. Huang X, Wei X, Sang T, Zhao Q, Feng Q, Zhao Y, Li C, Zhu C, Lu T, Zhang Z, Li M, Fan D, Guo Y, Wang A, Wang L, Deng L, Li W, Lu Y, Weng Q, Liu K, Huang T, Zhou T, Jing Y, Li W, Lin Z, Bucker ES, Qian Q, Zhang QF, Li J, and Han B. (2010) 2010-wide association studies of 14 agronomic traits in rice landraces. Nat. Genet 42, 961-967.
    Pubmed CrossRef
  20. Hwang WY, Fu Yanfang, Reyon Deepak, Maeder ML, Kaini P, Sander JD, Joung K, Peterson RT, and Yeh JYJ. (2013) 2013 and Precise Zebrafish Genome Editing Using a CRISPR-Cas System. PLOS One 8, e68708.
  21. Holkers M, Maggio I, Liu J, Janssen JM, Miselli F, Mussolino C, Recchia A, Cathomen T, and Goncalves MA. (2013) 2013 integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells. Nucliec Acids Res 1, e63.
  22. Hu B, Wang W, Ou S, Tang J, Li H, Che R, Zhang Z, Chai X, Wang H, Wang Y, Liang C, Liu L, Piao Z, Deng Q, Deng K, Xu C, Liang Y, Zhang L, Li L, and Chu C. (2015) 2015 in NRT1.1B contributes to nitrate-use divergence between rice subspecies. Nat. Genet 47, 834-838.
    Pubmed CrossRef
  23. Ikeda A, Ueguchi-Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, and Yamaguchi J. (2001) 2001 rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height- regulating gene GAI/RGA/RHT/D8. Plant Cell 13, 999-1010.
    Pubmed KoreaMed CrossRef
  24. Jain M. (2015) 2015 genomics of abiotic stress tolerance in plants:a CRISPR approach. Front. Plant Sci 6, 375.
  25. Jiang W, Bikard D, Cox D, Zhang F, and Marraffini LA. (2013) 2013-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31, 233-239.
    Pubmed KoreaMed CrossRef
  26. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, and Charpentier E. (2012) 2012 programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-21.
    Pubmed CrossRef
  27. Khatodia S, Bhatotia K, Passricha N, Khurana SMP, and Tuteja N. (2016) 2016 CRISPR/Cas Genome-EditingTool:Application in Improment of Crops. Frontiers in Plant Science 7, 506.
    CrossRef
  28. Kim YG, Cha J, and Chandrasegaran S. (1996) 1996 restriction enzymes:zinc finger fusions to Fok I cleavage domain. Proc Natl Acad Sci USA 93, 1156-60.
    Pubmed KoreaMed CrossRef
  29. Komor AC, Kim YB, Packer MS, Zuris JA, and Liu DR. (2016) 2016 editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533, 420-426.
    Pubmed KoreaMed CrossRef
  30. Kuscu C, Arslan S, Singh R, Thorpe J, and Adli M. (2014) 2014- wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nature Biotechnol 32, 677-683.
    Pubmed CrossRef
  31. Li Z, Liu ZB, Xing A, Moon BP, Koellhoffer JP, Juang L, Ward RT, Clifton E, Falco SC, and Cigan AM. (2015) 20159-guide RNA directed genome editing in soybean. Plant Physiology 169, 960-970.
    Pubmed KoreaMed CrossRef
  32. Lowder L, Malzahn A, and Qi Y. (2016) 2016 Evolution of Manifold CRISPR Systems for Plant Genome Editing. Front. Plant Sci 7, 1683.
    CrossRef
  33. Lu Y, and Zhu JK. (2016) 2016 editing of a target base in the rice genome using a modified CRISPR/Cas9. Molecular Plant 10, 523-525.
    Pubmed CrossRef
  34. Luo M, Wu X, Morbitzer R, Lahaye T, and Ayliffe M. (2017). 2017 Editing in Cereals , pp.W264. Proc. of the Plant and Animal Genome Conference (PAG XXV), an Diego, CA, USA.
  35. Ma Y, Zhang X, Shen B, Lu Y, Chen W, Ma J, Bai L, Huang X, and Zhang L. (2014) 2014 rats with conditional alleles using CRISPR/Cas9. Cell Research 24, 122-125.
    CrossRef
  36. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, and Church GM. (2013) 2013-guided human genome engineering via Cas9. Science 339, 823-826.
    Pubmed KoreaMed CrossRef
  37. Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, and Ikawa M. (2014) 2014 for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. Dev Growth Differ 56, 122-129.
    CrossRef
  38. Nakashima K, Ito Y, and Yamaguchi-Shinozaki K. (2009) 2009 regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol 149, 88-95.
    Pubmed KoreaMed CrossRef
  39. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Bi Y, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X, Ji W, and Sha J. (2014) 2014 of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 156, 836-843.
  40. Puchta H, and Fauser F. (2014) 2014 nucleases for genome engineering in plants:prospects for a bright future. Plant J 78, 727-741.
    Pubmed CrossRef
  41. Rouet P, Smih F, and Jasin M. (1994) 1994 of doublestrand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease. Mol. Cell Biol 14, 8096-8106.
    Pubmed KoreaMed CrossRef
  42. Sander JD, and Joung JK. (2014) 2014-Cas syetms for editing, regulating and targeting genomes. Nature Biotechnology 32, 347-355.
    CrossRef
  43. Shan Q, Wang Q, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, and Gao C. (2013) 2013 genome modification of crop plants using a CRISPR/Cas system. Nature Biotechnology 31, 686-688.
    Pubmed CrossRef
  44. Sternberg SH, Redding S, Jinek M, Greene EC, and Doudna JA. (2014) 2014 interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature 507, 62-67.
    Pubmed KoreaMed CrossRef
  45. Sun Y. (2017). 2017/Cas9-Mediated Gene Targeting for Crop Improvement , W731.
  46. Svitashev S, Young JK, Schwartz C, Gao H, Falco SC, and Cigan AM. (2015) 2015 mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and Guide RNA. Plant Physiology 169, 931-945.
    Pubmed KoreaMed CrossRef
  47. Voytas DF, and Gao C. (2014) 2014 genome engineering and agriculture:opportunities and regulatory challenges. PLoS Biol 12, e1001877.
    CrossRef
  48. Wang Y, Cheng X, Shan Q, Zhang Y, Liu J, Gao C, and Qiu JL. (2014) 2014 editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nature Biotechnology .
    CrossRef
  49. Wang S, Zhang S, Wang W, Xiong X, Meng F, and Cui X. (2015) 2015 targeted mjtageneis in potato by the CRISPR/Cas9 system. Plant Cell Rep 34, 1473-1476.
  50. Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu YG, and Zhao K. (2016) 2016 rice blast resistance by CRISPR/Cas9-targeted mutagenesis of the ERF transcription factpr gene OsERF922. PLOS One 11, e0154027.
    CrossRef
  51. Wolfe SA, Nekludova L, and Pabo CO. (2000) 2000 Recognition by Cys2His2Zinc finger proteins. Annu. Rev, Biphys. Biomol. Struct 3, 183-212.
    Pubmed CrossRef
  52. Yang D, Xu J, Zhu T, Fan J, Lai L, Zhang J, and Chen YE. (2014) 2014 gene targeting in rabbits using RNA-guided Cas9 nucleases. J Mol Cell Biol 6, 97-99.
    Pubmed KoreaMed CrossRef
  53. Zhang H, Zhang J, Wei P, Zhang B, Gou F, Feng Z, Mao Y, Yang L, Zhang H, Xu N, and Zhu JK. (2014) 2014 CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation. Plant Biotechnology Journal 12, 797-807.
    Pubmed CrossRef
  54. Zhu J, Song N, Sun S, Yang W, Zhao H, Song W, and Lai J. (2015) 2015 and inheritance of targeted mutagenesis in maize using CRISPR-Cas9. Journal of Genetics and Genomics 43, 25-36.
    Pubmed CrossRef


September 2017, 44 (3)
Full Text(PDF) Free

Social Network Service
Services

Cited By Articles
  • CrossRef (0)

Funding Information
  • CrossMark
  • Crossref TDM